木霉(Trichoderma sp.)HR-1活细胞吸附Pb(Ⅱ)的机理  

The mechanism of Pb(Ⅱ) sorption by living cell of Trichoderma sp.HR-1.

作　　者：沈薇[1] 杨树林[1] 李校堃[1] 袁辉[1] 高力虎[1] 

机构地区：[1]南京理工大学生物工程研究所,江苏南京210094

出　　处：《中国环境科学》2006年第1期101-105,共5页China Environmental Science

基　　金：国家"十五"重大科技专项(2004AA2Z3C60);南京市科技发展计划项目资助(200301045)

摘　　要：从重金属废水中筛选得到一株能够吸附Pb(II)的丝状真菌,编号为HR-1,初步鉴定其为木霉属(Trichodermasp.).研究了pH值对活细胞吸附铅离子能力的影响及其对铅的耐受性,结果表明,该细胞对铅离子的最大吸附量为597mg/g;扫描电镜(SEM)显示细胞吸附Pb(II)后发生明显的萎缩现象,在细胞表面存在大量的沉积物;X射线光散射能谱(EDX)验证了铅的吸附,同时吸附过程中存在离子交换作用.对比细胞吸附Pb(II)前后的傅立叶红外光谱(FTIR)图谱表明,葡聚糖或几丁质中的羟基和C-O-C、蛋白质的羧基是铅的主要吸附位点,与含氮官能团无关.XPS证实细胞吸附铅离子后生成PbO,XRD图谱得出其处于无定形和晶体之间.One filamentous fungus with high Pb（ Ⅱ ） biosorption ability was isolated from heavy metal wastewater and numbered HR-1, through primary identification it belonged to Trichoderma sp. The effect of pH value on biosorption by riving cell and its resistance to Pb（ Ⅱ ） was investigated. The maximum biosorption capacity of Pb（ Ⅱ ） by Trichoderma sp. HR-1 was 597mg/g. The precipitation on the cell wall was studied using combined technique of scanning electron microscopy （SEM） observation and energy dispersive X-ray analysis （EDX） showing week ion change and cell atrophied with lead deposits on its surface. The fourier transform infrared （FTIR） spectroscopy on blank and lead-loaded biomass demonstrated that the active groups such as the groups containing oxygen, e.g, hydroxyl and C-O-C in glucan or chitin and carboxyl group in protein on the cell wall seemed to be the main binding sites but nothing to do with nitrogen-containing groups. X-ray photoelectron spectra （XPS） showed that after biosorption PbO was formed with its form between amorphism and crystal by X-ray powder diffraction （XRD） detection.

关 键 词：细胞 生物吸附 SEM/EDX FTIR 吸附机理 

分 类 号：X172[环境科学与工程—环境科学]