肠内营养对梗阻性黄疸幼鼠肠屏障功能损害的保护作用  

Protective effects of enteral nutrition on gut barrier in immature rats with obstructive jaundice

作　　者：沙树贞[1] 李保文[1] 谢昌华[1] 傅廷亮[1] 张玉武 田洪根 

机构地区：[1]滨州医学院附属医院,山东滨州256603 [2]滨州市药品检验所

出　　处：《山东医药》2006年第9期15-16,共2页Shandong Medical Journal

基　　金：山东省中医药科技发展计划项目(2005-002)

摘　　要：目的探讨肠内营养对梗阻性黄疸(OJ)幼鼠肠屏障损害的保护作用。方法48只Wistar幼鼠随机分为正常对照组,假手术组,OJ组和OJ+能全素组。OJ+能全素组给予肠内营养10d,总热量为610kJ/(kg.d),氮量1.0g/(kg.d)。实验结束时检测其血清内毒素(LPS)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平,取腹腔液做细菌培养。结果OJ组血清LPS、TNF-α、IL-6水平显著高于正常对照组和假手术组,LPS、TNF-α水平显著高于OJ+能全素组,OJ+能全素组上述指标与正常对照组和假手术组间差异无显著性。对照组细菌培养阳性1例(1/12)、假手术组2例(2/10)、OJ组7例(7/8)、OJ+能全素组4例(4/8),OJ组与对照组、假手术组相比,P<0.05,差异有显著性。结论肠内营养有助于减轻OJ幼鼠肠屏障功能的损害。Objective： To evaluate the protective effects of enteral nutrition on intestinal barrier in immature rats with obstructive jaundice （OJ）. Methods： 48 Wistar rats were randomly divided into normal control group （N）; sham operation group! OJ group and OJ＋Nutrison group. In OJ＋Nutrison group, nutritional formulae were given for 10 days,total calorie was 610kJ/（kg. d） and nitrogen amount was 1.0g/（kg. d）. Serum endotoxin （LPS）, tumor necrosis factor-α（TNF-α）, interluekin-6 （IL-6） and bacteria culture of peritoneum fluid were detected. The intestinal mucosal structure was also observed. Results： LPS, TNF-α, IL-6 level were higher in OJ group than those in N group and sham operation guoup. LPS and TNF-α level were significantly higher than those in OJ＋Nutrison guoup. The LPS , TNF-α, IL6 level had no significant difference among OJ tnutrison guoup, N group and shan openation group. One bacteria cultrre was posirtve from 12 in N group , 2 from 10 in sham operation group, 7 from 8 im OJ group and 4 from 8 in OJ＋nutrison group. There was a significant difference between OJ group and N gvoup sham operation group （ P 〈0. 05）. Conclusion： The enteral nutrition can improve gut barrier function in immature rats with OJ.

关 键 词：肠屏障 肠内营养 梗阻性黄疸 保护作用 

分 类 号：R575.7[医药卫生—消化系统] R392.11[医药卫生—内科学]