蜈蚣藻多糖的提取分离及抗病毒活性的研究  

The Study on the EXtraction and the Antivirus Activity of Amylose Extracted from Grateloupia filicina

作　　者：朱艳梅[1] 王一飞[1] 张美英[1] 朱良[1] 康琰琰[1] 门晓媛[1] 岑颖洲[2] 

机构地区：[1]暨南大学生物医药研究开发基地,广东广州510632 [2]暨南大学化学系,广东广州510632

出　　处：《中药材》2006年第3期256-259,共4页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金团队项目(粤基办[2003]11号);广东省科技攻关项目(2003c50301);广州市科技攻关计划项目(2005Z3-e0411);2006年广东省工业攻关计划项目(2005A10904001)

摘　　要：目的:提取分离蜈蚣藻多糖,在体外检测不同浓度蜈蚣藻对单纯疱疹病毒Ⅱ型的抑制作用。方法:用五种不同的提取分离方法从蜈蚣藻中提取硫酸多糖,再从药物对细胞的保护、对HSV-2增殖的影响及对HSV-2感染细胞的综合作用三个方面研究不同浓度的蜈蚣藻抑制HSV-2对Vero细胞的感染作用,细胞病变效应法(CPE)观察和MTT法测定蜈蚣藻粗多糖抗HSV-2活性。结果:蜈蚣藻粗多糖能明显抑制HSV-2对Vero细胞的致病变作用,其中水提醇沉法所得的蜈蚣藻多糖的IC50为5.80μg/m。l结论:蜈蚣藻有较显著的抗HSV-2活性,且初步推测其抗HSV-2活性是作用在HSV-2和受体结合、吸附、侵入Vero细胞阶段,是一种具有潜在开发前景的药物。Objective： To determine the activity of anti-Herpes simplex Ⅱ virus（HSV-2） of amylose extracted from Grateloupiafilicina. Methods ： Grateloupia filicina amylose was extracted by five kinds of abstraction methods and its suppression on Herpes simplex Ⅱ virus was observed on cell level in three aspects ： the drug activity of protecting cell, the drug influence of HSV-2 proliferation and the drug synthesis action of HSV-2. Results： Grateloupiafilicina had prominence anti-Herpes simplex Ⅱ virus activity, IC50 of amylose extracted by water distilling and ethanol sedimentation was 5.80 μg/ml. Conclusion ： It suggest that the antivirus activity happen in the stage of HSV-2 binding, adsorption and ingression with Vero cell.

关 键 词：蜈蚣藻 细胞病变(CPE) MTF 

分 类 号：R285.5[医药卫生—中药学]