细胞因子TGF－β_1、PDGF－BB、bFGF在人肝纤维化中的作用  

ROLES OF CYTOKINES TGF-β_1, PDGF-BB AND bFGF IN HUMAN LIVER FIBROGENESIS

作　　者：徐冬波 翟为溶[1] 周筱梅[1] 庄丽[1] 张月娥[1] 

机构地区：[1]上海医科大学基础医学院病理学教研室,上海市肿瘤研究所生化和分子生物学室

出　　处：《上海医科大学学报》1996年第3期203-206,共4页Journal of Fudan University(Medical Science)

基　　金：自然科学基金

摘　　要：研究和探讨转化生长因子（ＴＧＦ）－β１、血小板源性生长因子（ＰＤＧＦ）－ＢＢ、碱性成纤维细胞生长因子（ｂＦＧＦ）在人肝纤维化中的作用，进一步阐明人体慢性肝病纤维化发生机制。外科切除的肝细胞标本按病变活动程度分为活动性、非活动性或轻度活动性以及对照组，以Ｎｏｒｔｈｅｒｎ分子杂交法测肝组织ＴＧＦ－β１ｍＲＮＡ，以免疫组化法显示组织原位ＰＤＧＦ－ＢＢ，ｂＦＧＦ及其相关细胞，并作半定量分析。结果：（１）肝病组织内ＴＧＦ－β１ｍＲＮＡ含量随肝病活动程度而增高（Ｐ＜０．０１）；（２）组织原位ＰＤＧＦ－ＢＢ、ｂＦＧＦ多肽定位及阳性细胞半定量显示，活动性肝病时两者均增高，阳性细胞所在部位与单核巨噬细胞以及胶原生成细胞一致，与Ⅰ、Ⅲ型胶原沉积也有密切关系。结论：本实验提示细胞因子ＴＧＦ－β１、ＰＤＧＦ－ＢＢ、ｂＦＧＦ在人肝纤维化中起着关键的调节作用。PURPOSE To study the roles of cytokines transforming growth factor(TGF)-β1,platelet-derived growth factor (PDGF)-BB and basic fibroblast growth factor (bFGF) in human hepatic fibrosis in order to make progress in investigation of liver fibrogenesis.METHODS Human liver tissue including active chronic liver diseases(ACLD), non-or mild-ACLD and considerably normal tissues were detected for TGF-β1 mRNA by Northern blot hybridization and localized for PD GF-BB,bFGF and their relateted cells by immunohistochemistry (IHC).RESULTS Northern Blot hybridization analysis showed that the amount of TGF-β1 mRNA was higher in chronic liver disease particularly in the ACLD. IH C revealed that PDGF-BB and bFGF positive staining were strong in ACLD, and their number and localization was associated with Lz and CD3 (markers for monocytes/macrophages and Pan T lymphocyte respectively) positive cells. The quantity and distribution of PDGF-BB and bFGF positive cells were coincided with extracellular matrix-producing cells (marked by desmin,a-smooth muscle actin and procollagen Ⅲ peptide), and as well as the Collagen Ⅰ and Collagen Ⅲ.CONCLUSIONS This study suggests that cytokines TGF-β1,PDGF-BB and bFGF may play important regulary roles in human liver fibrogenesis.

关 键 词：肝纤维化 细胞因子 PDGF BFGF 病理 

分 类 号：R575.202[医药卫生—消化系统]