光量子疗法对1型糖尿病新发患儿的免疫学疗效  

The immunological effect of photopheresis in children with newly diagnosed type 1 diabetes

作　　者：Faresj M.K. Ernerudh J. Berlin G. 刘凯 

机构地区：[1]Clinical Research Center, Division of Pediatrics, Faculty of Health Sciences, S-581 85 Linkping, Sweden Dr.

出　　处：《世界核心医学期刊文摘（儿科学分册）》2006年第2期55-56,共2页

摘　　要：Photopheresis has been claimed to have immune-modulat-ing effects, but the mechanisms of action are unknown. This study investigated the immune effect of photopheresis in children with type 1 diabetes, with a focus on the balance of Th1-and Th2-like cytokines. Ten children with newly diagnosed type 1 diabetes (10-17 y) were treated with five double treatments of photopheresis and 10 children matched for disease, age, and gender were given placebo tablets and sham pheresis. Expression of IFN-γ and IL-4 mRNA was determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and secretion of IFN-γ , IL-10, and IL-13 in cell-culture supernatants by ELISA after stimulation with glutamic acid decarboxylase (GAD65) (a.a. 247-279), the ABBOS peptide (a.a. 152-169), insulin, phytohemagglutinin (PHA), and keyhole limpet hemocyanin (KLH). Photopheresis changed antigen-stimulated immune balance in line with a Th2-like shift. Thus, the ratio of IFN-γ /IL-4 mRNA expression after in vitro stimulation with a peptide of the autoantigen GAD 65 was reduced after treatment in the photopheresis group. The IFN-γ /IL-4 mRNA expression ratio after in vitro stimulation with insulin was also lower in children treated with photopheresis compared with the placebo group. Photopheresis has an immune-modulating effect in children with type 1 diabetes, causing a Th2-like deviation.Photopheresis has been claimed to have immune-modulating effects, but the mechanisms of action are unknown. This study investigated the immune effect of photopheresis in children with type 1 diabetes, with a focus on the balance of Th1 - and Th2 - like cytokines. Ten children with newly diagnosed type 1 diabetes （10 - 17 y） were treated with five double treatments of photopheresis and 10 children matched for disease, age, and gender were given placebo tablets and sham pheresis. Expression of IFN -γ and IL-4 mRNA was determined by real-time reverse-transcriptase polymerase chain reaction （RT-PCR） and secretion of IFN-% IL-10, and IL-13 in cell-culture supematants by ELISA after stimulation with glutamic acid decarboxylase （GAD65） （a. a. 247-279）, the ABBOS peptide （a. a. 152 - 169）, insulin, phyto- hemagglutinin （PHA）, and keyhole limpet hemocyanin （KLH）.

关 键 词：光量子疗法 1型糖尿病 免疫学 实时逆转录聚合酶链反应 患儿 疗效 ELISA法检测 mRNA表达 INF-γ 光量子治疗 

分 类 号：R743.330.5[医药卫生—神经病学与精神病学] R587.1[医药卫生—临床医学]