CSF-1对体外培养人牙囊细胞OPG表达的影响  

Regulation of osteoprotegerin expression by CSF-1 in human dental follicle cells

作　　者：孙海燕[1] 林珠[1] 金作林[1] 张永宽[1] 

机构地区：[1]西安第四军医大学口腔医学院正畸科,710032

出　　处：《实用口腔医学杂志》2006年第2期214-217,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(30400510)

摘　　要：目的：研究集落刺激因子（CSF．1）对体外培养的人牙囊细胞骨保护素（OPG）表达的影响。方法：原代培养人牙囊细胞，传代至第3代，制备细胞爬片，进行OPG免疫组化染色；3～6代人牙囊细胞长至80％时，用胰酶消化，离心，重悬，制成单细胞悬液，接种到细胞培养皿，细胞长满至80％时，培养基中加入25ng／ml的CSF-1，孵育0．5、1、3、6h，分别收集上清液，ELISA法检测OPG蛋白分泌量的变化，提取总RNA进行RT-PCR，检测OPGmRNA的表达变化。结果：正常人牙囊细胞OPG蛋白表达阳性；25ng／ml的CSF-1可降低人牙囊细胞OPG的表达，共同孵育1h，OPG表达降至最低（P〈0．05）。结论：牙囊细胞表达OPG，同时在牙齿萌出的特定时期，CSF-1通过下调人牙囊细胞OPG，减弱对破骨细胞形成的抑制作用，促进破骨细胞分化成熟，调节牙齿萌出。Objective：To study the effects of colony-stimulating factor-1 （ CSF-1 ） on the expression of osteoprotegerin （ OPG ） in human dental follicle cells. Methods： Human dental follicle cells culture was established and cell crawling slides were stained by OPG antibody. The third to sixth passage ceils were incubated with 25 ng/ml CSF-1 for 0. 5,1,3 and 6 h respectively, then the conditioned medium and cells were collected for ELISA and RT- PCR assay respectively. Results：OPG was expressed in the cytoplasm of human dental follicle cells. GSF-1 at 25 ng/ml and 1 h-exposure down-regulated OPG expression in the ceils（ P 〈 0. 05 ）. Conclusion： OPG is located in human dental follicle cells,GSF-1 may down-regulate OPG expression in the cells.

关 键 词：牙囊 集落刺激因子 骨保护素 牙齿萌出 

分 类 号：R780.2[医药卫生—口腔医学]