Deguelin inhibits expression of IκBα protein in Raji and U937 cells  

作　　者：Wei-hua CHEN Yan CHEN Guo-hui CUI 

机构地区：[1]Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

出　　处：《Acta Pharmacologica Sinica》2006年第4期485-490,共6页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(№30472267).

摘　　要：Aim： To determine whether deguelin can regulate the expression of nuclear factor kappa B （NF-κB） binding protein （IκBα） in U937 human leukemia cells and Raji human B lymphoma cells. Methods： The localization of IκBα protein was investigated by using an immunofluorescence method. The expression of Ird3a and NF- κB/p65 proteins in Raji and U937 cells were investigated by using Western blotting. Apoptosis was detected through annexin V/PI double-labeled cytometry. Results： IκBα localized in the cytoplasm in untreated and deguelin-treated cells. After treatment with tumor necrosis factor a （TNF-α） or deguelin plus TNF-α for 15 min, there was a substantial reduction in the amount of IκBα protein. The expression of IκBα was downregulated by deguelin in Raji and U937 cells. Deguelin induced apoptosis in U937 cells. Conclusion： Deguelin inhibited the expression of IκBα protein in U937 and Raji cells. The anti-proliferative activity of deguelin is related to the signal pathway of NF-κB.Aim： To determine whether deguelin can regulate the expression of nuclear factor kappa B （NF-κB） binding protein （IκBα） in U937 human leukemia cells and Raji human B lymphoma cells. Methods： The localization of IκBα protein was investigated by using an immunofluorescence method. The expression of Ird3a and NF- κB/p65 proteins in Raji and U937 cells were investigated by using Western blotting. Apoptosis was detected through annexin V/PI double-labeled cytometry. Results： IκBα localized in the cytoplasm in untreated and deguelin-treated cells. After treatment with tumor necrosis factor a （TNF-α） or deguelin plus TNF-α for 15 min, there was a substantial reduction in the amount of IκBα protein. The expression of IκBα was downregulated by deguelin in Raji and U937 cells. Deguelin induced apoptosis in U937 cells. Conclusion： Deguelin inhibited the expression of IκBα protein in U937 and Raji cells. The anti-proliferative activity of deguelin is related to the signal pathway of NF-κB.

关 键 词：DEGUELIN IΚBΑ nuclear factor kappa B tumor necrosis factor-α APOPTOSIS Raji cells U937 cells 

分 类 号：R9[医药卫生—药学]