P120ctn overexpression enhances β-catenin-E-cadherin binding and down regulates expression of survivin and cyclin D1 in BEL-7404 hepatoma cells  

作　　者：Chao-Zan Nong Li-Li Pan Wei-Sheng He Xi-Liang Zha Hai-HongYe Hua-Yi Huang 

机构地区：[1]Department of Experimental Center, Guangxi Hospital tor Nationalities, Nanning 530001, Guangxi ZhuangAutonomous Region, China [2]Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai200032, China

出　　处：《World Journal of Gastroenterology》2006年第8期1187-1191,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.30160096;Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.0007037 and No.0342020

摘　　要：AIM:To understand the role of P120ctn in E-cadherin-mediated cell-cell adhesion and signaling as well as inhepatoma cell biological function.METHODS:We stably overexpressed p120ctn isoform3A in BEL-7404 human hepatoma cells and studied theeffect of p120ctn on β-catenin and E-cadherin bindingas well as p120ctn and β-catenin subcellular localizationusing immunoprecipitation,Western blotting and confocalmicroscopy.We also investigated the inhibitory effectof p120ctn transfection on the expression of apoptoticprotein survivin survivin and cell cycle regulator cyclin D1in the cells.RERULTS:Western blotting indicated that p120ctnexpression increased after cells were transfected withp120ctn isoform 3A.The protein was located mainlyat membrane under immunofluorescent microscope.β-catenin nuclear expression was reduced afteroverexpression of p120ctn isoform 3A.The p120ctn-E-cadherin binding increased after transfection of p120ctnisoform 3A.Furthermore,overexpression of p120ctndown regulated the expression of apoptotic protein sur-vivin and cell cycle regulator cyclin D1.These effects ledto reduction of cell proliferation.CONCLUSION:Our results indicate that p120ctnplays an important role in regulating the formation ofE-cadherin and-catenin complex,cell apoptosis,cellcycle and cancer cell biological function.AIM： To understand the role of P120ctn in E-cadherinmediated cell-cell adhesion and signaling as well as in hepatoma cell biological function. METHODS： We stably overexpressed p120ctn isoform 3A in BEL-7404 human hepatoma cells and studied the effect of p120ctn on β-catenin and E-cadherin binding as well as p120ctn and β-catenin subcellular localization using immunoprecipitation, Western blotting and confocal microscopy. We also investigated the inhibitory effect of p120ctn transfection on the expression of apoptotic protein survivin survivin and cell cycle regulator cyclin D1 in the cells. RERULTS： Western blotting indicated that p120ctn expression increased after cells were transfected with p120ctn isoform 3A. The protein was located mainly at membrane under immunofluorescent microscope. β-catenin nuclear expression was reduced after overexpression of p120ctn isoform 3A. The p120ctn-Ecadherin binding increased after transfection of p120ctn isoform 3A. Furthermore, overexpression of p120ctn down regulated the expression of apoptotic protein survivin and cell cycle regulator cyclin D1. These effects led to reduction of cell proliferation. CONCLUSION： Our results indicate that p120ctn plays an important role in regulating the formation of E-cadherin and -catenin complex, cell apoptosis, cell cycle and cancer cell biological function.

关 键 词：P120CTN β-catenin Hepatoma Cyclin D1 SURVIVIN 

分 类 号：R735.7[医药卫生—肿瘤]