Survivin siRNA协同5-FU抑制MCF-7细胞的增殖(英文)  

Synergism between a siRNA targeted to survivin and 5-FU in inhibiting MCF-7 cell proliferation in vitro

作　　者：张淑群[1] 张淑慧[2] 薛兴欢[1] 王西京[1] 姜建涛[1] 

机构地区：[1]西安交通大学第二医院肿瘤外科,陕西西安710004 [2]西北工业大学化学工程系,陕西西安710072

出　　处：《南方医科大学学报》2006年第3期251-254,共4页Journal of Southern Medical University

基　　金：Supported by National Natural Science Foundation of China(30500600)and Key Sci-Tech Research Project of Shaanxi Province(2003k10-G41)

摘　　要：目的研究以survivin为靶标的小干扰RNA(siRNA)与化疗药5-FU联合应用抑制MCF-7细胞增殖的作用。方法利用T7 RNA聚合酶体外转录合成siRNA并筛选出一条RNA干扰survivin基因的靶序列。以脂质体为载体,将survivin siRNA转染至人乳腺癌MCF-7细胞中,用四氮唑盐(MTT)法染色并计算siRNA联用5-FU对MCF-7细胞的抑制率,用SAS统计软件及金正均Q值法进行统计分析。结果单用5-FU处理细胞,其IC50为4.42μg/ml,而加入5 nmol/L siRNA后,其IC50降为1.18μg/ml,siRNA与5-FU联用对MCF-7细胞的抑制作用较单用5-FU明显增强(F=26.74,P<0.01);Q值分析表明survivin siRNA与中低浓度的5-FU联用,有较好的协同作用(Q≥1.15)。结论survivinsiRNA可显著增强5-FU对MCF-7细胞增殖的抑制,提高肿瘤细胞对化疗药物的敏感性,克服耐药性的产生。Objective To investigate the role of small interfering RNA （siRNA） targeted to survivin in combination with 5-fluorouracil （5-FU） in inhibiting the proliferation of MCF-7 cells. Methods A siRNA targeted to survivin was synthesized and transfected into MCF-7 cells via lipofectin. Changes of the cell growth activity in response to combined treatment with survivin siRNA and 5-FU or 5-FU treatment alone was evaluated by MTT assay. The Q method of Jin 7：henjun was used to evaluated synergism between the synthesized siRNA and 5-FU. Results Treatment with 5 nmol/L siRNA reduced the IC50 of 5-FU from 4.42 to 1.18μg/ml, and the inhibitory effect of combined treatment on MCF-7 cells was higher than that of 5-FU alone （F=26.74, P〈0.01）. Synergism effect （Q≥ 1.15） was observed between 5-FU at lower concentrations and survivin siRNA. Conclusion siRNA may enhance the effectiveness of 5-FU in inhibiting the proliferation of MCF-7 cells.

关 键 词：RNA干扰 化学疗法 联合治疗 MCF-7细胞 

分 类 号：R737.9[医药卫生—肿瘤]