酸性成纤维细胞生长因子预防失神经支配肌运动终板退变的形态学及电生理研究  

Acidic fibroblast growth factor for preventing motor endplate degeneration and muscular atrophy after motor nerve injury: a morphological and electrophysiological study

作　　者：杨绍安[1] 靳安民[1] 邹小英[1] 肖晓桃 肖莎[1] 

机构地区：[1]南方医科大学珠江医院骨科,广东广州510282 [2]广州市石溪中医院,广东广州510288

出　　处：《南方医科大学学报》2006年第3期313-315,共3页Journal of Southern Medical University

基　　金：广东省医学科研基金(A2003472)~~

摘　　要：目的本研究旨在探讨运动神经损伤后运动终板退变的预防方法。方法SD大鼠30只,等分成3组。处理组切断右侧腓总神经,修复神经,酸性成纤维细胞生长因子(aFGF)纤维蛋白凝胶载体植入失神经支配的胫前肌神经区周围的肌间隙,设立对照。观察6周,进行形态学及电生理检查。结果采用aFGF纤维蛋白凝胶载体组,运动终板结构基本正常,肌肉无明显萎缩;对照组运动终板明显退变或消失,肌肉明显萎缩;采用aFGF纤维蛋白凝胶载体组低频重复电刺激(RNS)衰减率明显低于对照组。结论aFGF纤维蛋白凝胶载体可以有效保护失神经支配肌运动终板,防止肌肉萎缩,促进神经肌肉接头功能的恢复。Objective To explore measures to prevent motor endplate degeneration and muscular atrophy after motor nerve injury. Methods Thirty Sprague-Dawley rats were randomized into 3 equal groups. In two of the groups, the right common peroneal nerves of the rats were transected and immediately sutured with implantation of collagen gel carrier of acidic fibroblast growth factor （aFGF） or the empty carrier into the denervated tibialis anterior muscles. In the control group, the transected nerves were sutured without implantation. Six weeks after the operation, morphological and electrophysiological examinations were performed. Results In the control rats and those with empty collagen gel carrier implantation, obvious motor endplate degeneration and muscular atrophy occurred, which were not obvious in rats receiving aFGE carrier implantation. The decrement of repetitive nerve stimulation was significantly greater in the former two groups than in the latter. Conclusion Implantation of collagen gel carrier of aFGF may prevent motor endplate degeneration and facilitate functional recovery of the neuromuscular junction after motor nerve injury.

关 键 词：运动神经损伤 酸性成纤维细胞生长因子 运动终板 纤维蛋白凝胶 重复电刺激 

分 类 号：R361[医药卫生—病理学]