不同频率电磁场对胆囊癌细胞增殖及DNA损伤作用  

Effects of electromagnetic fields of different frequencies on proliferation and DNA damage of gallbladder cancer cells

作　　者：陈平[1] 杨耀琴[2] 陶惠红[2] 杨虎川[2] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]同济大学医学院肿瘤细胞研究室,上海200092

出　　处：《南方医科大学学报》2006年第3期328-330,共3页Journal of Southern Medical University

基　　金：上海市科委基金(1509236001)~~

摘　　要：目的探讨不同频率的低功率电磁场对胆囊癌细胞恶性增殖及DNA损伤的作用及其意义。方法采用绘制细胞生长曲线及单细胞凝胶电泳技术分别检测经不同频率电磁场作用后,胆囊癌细胞的增殖及DNA损伤情况。结果经不同频率(0.1￣40 MHZ)电磁场作用后,各实验组总体上均可见细胞增殖受到抑制,DNA链产生断裂损伤,统计学检验较正常对照有显著意义。结论0.1￣40 MHZ范围低功率电磁场对胆囊癌细胞增殖及DNA损伤产生明显影响,主要表现为使DNA链产生断裂损伤,进一步抑制细胞增殖,且这种作用和频率有关,但并不是“线性”关系。Objective To study the effects of low-power electromagnetic fields of different frequencies on proliferation and DNA damage of gallbladder cancer cells. Methods The cell growth curve was drawn and single cell gel electrophoresis performed to evaluate the proliferation and DNA damage of gallbladder cancer cells respectively after the cells were exposed to electromagnetic fields of different frequencies. Results After exposure to low-power electromagnetic fields of different fre- quencies （0.1-40 MHz）, the cells displayed significant changes with obvious cell proliferation inhibition and DNA strand breakage. Conclusion Low-power electromagnetic fields within the range of 0.1-40 MHz may impair the DNA strand and cause inhibition of proliferation of the gallbladder cancer cells, and these effects are related to the frequency of the electromag- netic fields but not in a linear fashion.

关 键 词：频率 电磁场 单细胞凝胶电泳 细胞培养 胆囊肿瘤 DNA损伤 

分 类 号：R735.8[医药卫生—肿瘤]