胰岛素样生长因子Ⅰ型受体单克隆抗体对结肠癌细胞生长的影响  

The effect of IGF- I receptor monoclonal antibody on human colorectal cancer cell lines

作　　者：张亚武[1] 张有成[1] 张小明 寇治民[1] 李徐生[1] 

机构地区：[1]兰州大学第二医院普外科 [2]甘肃省庆阳市第一人民医院

出　　处：《中华普通外科杂志》2006年第3期200-202,共3页Chinese Journal of General Surgery

基　　金：甘肃省科技攻关项目(2GS042-A43-014-14)

摘　　要：目的探讨胰岛素样生长因子I型受体(insulin-like growth factor I receptor,IGF-I R) 在人结肠癌细胞株HT-29上的表达,以及两种胰岛素样生长因子I型受体单克隆抗体(IGF-I R McAb)对HT-29细胞增殖的影响。方法免疫组织化学法检测HT-29的IGF-I R表达,MTT法检测两种IGF-I R McAb对HT-29的抑制增殖作用及诱导凋亡情况。结果人结肠癌细胞株HT-29 细胞膜高表达IGF-I R。IGF-I R McAb能抑制HT-29细胞增殖,McAb对HT-29细胞的抑制作用随抗体浓度增大而增大。IGF-I R McAb诱导HT-29凋亡,与对照组相比差异有统计学意义 (P<0．01)。结论IGF-I R McAb通过阻断IGF-I R抑制结肠癌细胞增殖、诱导细胞凋亡。Objective To explore the expression of insulin-like growth factors and their receptor （ IGF- Ⅰ R） in a human colorectal cancer cell line HT-29, and the effect of two IGF- Ⅰ R monoclonal antibodies on the biological behavior of HT-29 cell lines. Methods Immunohistochemistry was used to test the expression of IGF- Ⅰ R in HT-29 cell lines. MTF assay was used to determine the anti-proliferation effects of IGF-Ⅰ R monoclonal antibody （McAb）. Apoptosis percentage was estimated by flow cytometry （FCM）. Results IGF- Ⅰ R was expressed in the membranes of HT-29 cell lines. McAb of IGH-Ⅰ R can inhibit the proliferation of HT-29 cell lines in a dose-dependent manner. The apoptosis rate of HT-29 cells treated by IGF-ⅠR McAb was higher than that of control group （ P 〈 0. 01 ）. Conclusions Blocking IGF-ⅠR, IGF-ⅠR McAb inhibits proliferation and induces apoptosis of HT-29 cell lines.

关 键 词：结肠肿瘤 受体 IGF1型 抗体 单克隆 

分 类 号：R735.35[医药卫生—肿瘤]