猪骨骼肌缺血预适应降低心肌缺血再灌注后基质金属蛋白酶的表达  

Remote preconditioning induced by skeletal muscle ischemia reduces matrix metalloproteinases expression in porcine myocardium

作　　者：谢瑞芹[1] 李荣芹[1] 崔炜[1] 郝玉明[1] 李保华[1] 吴金凤[1] 杜国英[1] 张涛[1] 刘凡[1] 

机构地区：[1]河北医科大学第二医院心内科,河北石家庄050000

出　　处：《基础医学与临床》2006年第3期280-283,共4页Basic and Clinical Medicine

基　　金：河北省科技攻关基金(0527610ID-55)

摘　　要：目的观察猪骨骼肌缺血预适应对心肌缺血再灌注后坏死面积及心肌MMP-2、MMP-9及TIMP-1的影响。方法10只小型猪被随机分为缺血再灌注(I/R)组和远端预适应(RP)组。采用非开胸法建立心脏I/R模型,通过球囊堵塞左股动脉造成骨骼肌短暂缺血。以三硝基四氮唑红确定心肌梗死范围;以免疫组化和RT-PCR法测I/R心肌中MMP-2、MMP-9和TIMP-1表达及骨骼肌RP对此的影响。结果与I/R组相比,骨骼肌RP明显减少心肌梗死范围。在缺血区,RP明显降低MMP-2和MMP-9的表达,增加TIMP-1表达(P<0.05)。且RP明显减弱MMP-2和MMP-9 mRNA的表达,增强TIMP-1的mRNA表达(P<0.05)。结论骨骼肌缺血预适应心肌不仅可减少心肌坏死范围,还可能对心肌间质产生保护作用。Objective To determine the effect of skeletal muscle ischemic preconditioning on myocardial infarct area and matrix metalloproteinases （MMPs） in porcine myocardium following ischemia resperfusion （I/R）. Methods Ten pigs were divided into I/R group and remote preconditioning （RP） group. The myocardial infarct size was measured with triphenyl tetrazolium choride （TIC）. MMP-2, MMP-9 and TIMP-1 of myocardium in different areas were observed by immunohistochemistry and RT-PCR. Results In I/R group, the myocardial infarct size, expressed as percentage of the ischemic myocardium was（51.1 ± 2.3） % and it was reduced to（24.1±1.5） % in RP group （ P 〈 0.05）. Immunohistochemical studies showed that the expression of MMP-2 and MMP-9 was higher, and TIMP-1 was lower in I/R group in ischemic area than that in non-ischemic area （ P 〈 0.05）. But expression of MMP-2 and MMP-9 decreased, and TIMP- 1 increased in RP group than that in I/R group （ P 〈 0.05）. RT-PCR studies showed that expression of MMP-2 and MMP-9 mRNA became stronger, and expression of TIMP-1 mRNA became weaker in ischemic area than nonischemic area in I/R group （ P 〈 0.05）. But expression of MMP-2 and MMP-9 mRNA became weaker, and expression of TIMP-1 mRNA became stronger in RP group than that in I/R group （ P 〈 0.05）. Conclusion RP induced by skeletal muscle isch-emia not only reduce mycardial infarct size, but also be potential protection of cardiac matrix from degrading.

关 键 词：骨骼肌 远端预适应 心脏保护 基质金属蛋白酶 

分 类 号：R363[医药卫生—病理学]