Fe(Ⅲ)-PAR络合物光还原分光光度法测定食用菌中微量铁  

Spectrophotometric determination of micro-iron in the fungus by the photoreduction of Fe(Ⅲ)-PAR complex

作　　者：吴小华[1] 缪吉根[1] 缪煜清[1] 陈建荣[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004

出　　处：《分析试验室》2006年第4期58-60,共3页Chinese Journal of Analysis Laboratory

基　　金：浙江省分析测试基金资助项目(98061)

摘　　要：研究了溶液酸度、光强度、光照时间及显色剂用量对Fe(Ⅲ)-PAR络合物光还原反应的影响。在pH 4.5 HAc-NaAc缓冲溶液中,PAR溶液浓度为1.11×10-3mol/L,250 W荧光高压汞灯照射40 min,Fe(Ⅲ)-PAR络合物光还原反应可趋于完全,据此建立光还原代替化学还原测定微量铁的新方法,该方法用于食用菌中微量铁的测定。The effects of solution acidity, light intensity, illuminating time and PAR amount on the photoreduction of Fe（ Ⅲ ）-PAR complex were studied. The results showed that in a HAc-NaAc buffer solution （pH 4.5） with the concentration of PAR 1.11 × 10^-3 mol/L, Fe（ Ⅲ ）-PAR complex, which was illuminated by the high-pressure fluorescent Hg-lamp （250 W） for 20 min, could be transformed into Fe（ Ⅲ ）-PAR complex completely. Based on the results, a new photoreduction method was developed to hopefully substitute the traditional chemistry reduction method for the determination of micro-iron. The new method was applied successfully in the determination of micro-iron in the fungus and satisfactory results were obtained.

关 键 词：铁 4-(2-吡啶偶氯)间苯二酚 光还原 分光光度法 食用菌 

分 类 号：O657.3[理学—分析化学]