磷酰化芳香族氨基酸的激光诱导荧光光谱  

Laser-induced Fluorescence of Phosphoryl Aromatic Amino Acids

作　　者：周义东[1] 周伟华[1] 赵玉芬[1] 赵朔嫣[1] 张培林[1] 

机构地区：[1]清华大学生命有机磷化学教委开放实验室

出　　处：《应用激光》1996年第5期196-198,共3页Applied Laser

基　　金：国家自然科学基金

摘　　要：利用Ｎｄ：ＹＡＧ激光器的四倍频输出２６６ｎｍ近紫外光激发固体纯磷酰化芳香族氨基酸样品，获得可见光波段的荧光发射，其光谱结构分别为芳香环和磷酰基的发射，结果发现取代基的导入使芳香环荧光峰红移，而对磷酰基的荧光峰的影响则为紫移。Visible fluorescence of pure solid N-phosphoryl aromatic amino acids has been achieved under the excitation of the output of frequency - quadrupled Nd: YAG Laser (266nm).The two peaks of fluorescence are assigned to the emission of the aromatic ring and the double bond of phosphoryl group. It was found that the substituent makes the emission of aromatic ring red shift,and the phosphoryl group blue shift.

关 键 词：磷酰化芳香族 氨基酸 蛋白质 荧光光谱 

分 类 号：Q631[生物学—生物物理学]