Ta1小麦不同轮选世代不育株的HMW-GS组成与分析  

Composition of HMW-GS of the Sterile Plants from Different Generations of Taigu-male-sterile Wheat Recurrent Selection Population

作　　者：刘正斌[1] 化斌[2] 高庆荣[1] 赵桂清 乔晓琳[1] 邱新民[1] 

机构地区：[1]山东农业大学农学院,山东泰安271018 [2]山东农业大学林学院,山东泰安271018 [3]阳谷县农业局,山东阳谷252300

出　　处：《麦类作物学报》2006年第2期72-76,共5页Journal of Triticeae Crops

基　　金：山东省自然科学基金项目(Y2002D08);山东省科技攻关项目[鲁科计字2003112号(031040113)]

摘　　要：为了给T a1小麦轮选育种提供一定的参考,利用SDS-PAGE方法对T a1小麦轮选群体C2、C5世代不育株及亲本的HMW-G S组成与变异进行分析。结果表明:(1)C2世代不育株的HMW-G S变异十分广泛,变异系数高达94.2%。31种亚基组合中“1,7+8,2+12”出现频率最高。G lu-B 1位点亚基变异最丰富,有15种类型,而且产生了7、22、13+16等亲本中不存在的亚基;G lu-D 1位点优质亚基5+10仅占18.0%,2+12亚基仍占明显优势。(2)C5世代不育株的HMW-G S变异较亲本广泛,29种亚基组合中,杂合的“1,7+8/14+15,5+10”出现频率最高(12.7%),其余皆小于7.0%;G lu-B 1位点变异类型仅有9种,且多为杂合。C5世代优质亚基5+10、14+15出现频率提高至40.0%和32.0%,但变异系数降为80.7%,表明轮选效果明显,但C5世代群体的遗传异质性较C2世代呈下降趋势。Compositions of HMW-GS of the sterile plants from different generations in Tal （Talgu-malesterile） wheat recurrent selection population was analyzed by SDS-PAGE. The results showed that： （1） Variance of HMW-GS in sterile plants in C2 generation was extremely wide with of CV% of 94.2 %. The eombination＂1,74.8,2＋12＂had the highest ratio 14.0% among the identified 31 ones. Among the 3 locus that controls glutenin, Glu-B1 which had 15 styles had the widest variance, with the recombinations of the new subunits 7,22,13＋16 that didn＇t exist in the parents. However, high quality subunits 5＋10 had a low ratio of 18.0%, and the subunits 2＋12 was still dominant. （2）Compared with parents, variance of HMW-GS in C5 generation was still wide. The converged ＂1,74-8/14＋15,5＋10＂subunits had the highest ratio （12.7%） among 29 identified combinations, the others were less than 7.0%. Only 9 styles of HMW-GS variation were observed in Glu-B1 locus, most were converged ones and the rare 13＋16 was not detected in this generation. Compared to Cz generation, hereditary heterogeneity of Cs population was declined. Ratio of high quality subunits 5 ＋10,14＋15 was enhanced to 40.0 %, 32.0 %, so the effect of recurrent selection was remarkable. Meanwhile, CV% was decreased to 80. 7%, the result indicated that hereditary heterogeneity of Tal wheat recurrent selection population tended to decline, some high quality subunits like 13 ＋16,17＋ 18, 7^oe etc should be put into the gene pool before the next generation was formed in order to make this population play the better role.

关 键 词：太谷核不育小麦 轮选群体 高分子量咎蛋白亚基 遗传异质性 

分 类 号：S512.1[农业科学—作物学] S331