SARS-CoV寡核苷酸基因芯片在SARS病例咽拭或咽漱液检测中的应用  

Application of SARS-CoV oligonucleotide microarray in detection of saliva samples in SARS patients

作　　者：黄吉城[1] 马文丽[1] 石嵘[1] 欧阳谦[1] 陈秋霞[2] 周珏宇[1] 丁大鹏[1] 郑文岭 

机构地区：[1]南方医科大学基因工程研究所,广州510515 [2]广东省疾病预防控制中心,广州510300 [3]华南基因组中心,广州510800

出　　处：《广东医学》2006年第4期483-485,共3页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2003FD01-05)

摘　　要：目的采用SARS-CoV寡核苷酸基因芯片对200份临床确诊SARS病例的咽拭或咽漱液、401例非SARS感染的呼吸道患者的咽拭或咽漱液样品进行检测,评价该方法用于SARS-CoV核酸检测的灵敏度、特异性及其应用于早期诊断的可能性。方法200例临床确诊SARS患者样品,401阴性对照样品采用双盲法进行测定,抽提可能存在的病毒RNA,采用限制性荧光标记法标记,在同等条件下,进行基因芯片杂交、清洗和芯片扫描检测。通过对杂交结果的数据分析,比照临床诊断和血清学的检测结果,对该方法的灵敏度和特异性及应用于SARS早期诊断的可能性进行评价。结果对601份标本检测的结果,灵敏度为71.50%,特异度为92.01%。对发病10 d内采集临床确诊SARS病例的标本,检测的灵敏度为81.08%。结论利用SARS-CoV寡核苷酸基因芯片检测SARS患者发病早期的咽漱液或咽拭子中的SARS-CoV核酸,灵敏度较高,可作为早期基因诊断。Objective To detect the virus nueleotide in 601 saliva samples（200 SARS patients and 401 other patients） collected clinically using DNA mieroarray and to evaluate its sensitivity, specificity and possibility for early diagnosis of SAILS. Method Saliva samples of 200 patients diagnosed as SAILS and 401 negative controls were prepared for double blind deteetian. Possible virus nueleofides were extracted and were labeled with RD labeling method. The labeled samples were applied to the mieroarray and were hybridized, washed, scanned and analyzed in parallel with the same parameters. The evaluation of sensitivity, specificity and possibility for early diagnosis were educed by data analysis and correlation with clinical and serology diagnostic resuits. Results The diagnostic sensitivity was 71.50%, while the specificity was 92.01%. The diagnostic sensitivity in early stage（ 〈 10 days） of the disease was 81.08%. Condusion DNA microarray can detect virus nuclcotide from saliva samples for early diagnosis. Its high sensitivity improves the detection rate of the disease.

关 键 词：SARS—CoV 基因芯片 限制性荧光标记 基因诊断 

分 类 号：R373.21[医药卫生—病原生物学] R19[医药卫生—基础医学]