人卵巢癌耐药细胞株的比较蛋白质组分析  

Comparative Analysis of Proteomes for Chemoresistance in Human Ovarian Adenocarcinoma Cell Line,COC_1/DDP

作　　者：吕磊[1] 刘志强[1] 李丽[1] 刘宁[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所新药研究实验室长春质谱中心

出　　处：《高等学校化学学报》2006年第4期635-637,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20273067;20173057);中国科学院知识创新工程(批准号:KGCX2-SW-213-06)资助

摘　　要：The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE. The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment. Approximate 1 300 protein spots were resolved in the two ranges of pl（3-10）. The spots which have 10-fold difference in light density were digested by in-gel enzyme, identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDITOF-MS） and finally detected by Profound and Mascot search engine in IDI databases. Seven proteins with obviously altered abundances were detected specifically, including four up-regulated protein spots and three down-regulated protein spots. The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.

关 键 词：卵巢癌 蛋白质组 质谱 

分 类 号：O629.73[理学—有机化学]