胸腺体液因子(THF-γ2)的合成和活性测定  

Synthesis and Activity Determination of Thymus Humoral Factor(THF-γ2)

作　　者：廖晓全[1] 赵宗阁[2] 周立娜[3] 徐康森[2] 袁勤生[1] 

机构地区：[1]华东理工大学生物工程学院生物反应器国家重点实验室,上海200237 [2]中国药品生物制品检定所,北京100050 [3]中国医科大学,沈阳110001

出　　处：《中国药学杂志》2006年第7期548-550,共3页Chinese Pharmaceutical Journal

摘　　要：目的化学合成胸腺体液因子(THF-γ2),并建立纯化、分析和活性测定的方法。方法采用Fmoc固相合成法(SPPS)合成了胸腺体液因子,用中压液相(MPLC)反相柱一步纯化,经HPLC分析纯度,通过质谱和氨基酸序列测定鉴定合成产物。根据THF-γ2促进T细胞产生IL-2的特性检测合成产物的体外活性。结果化学合成胸腺体液因子的产率为95.8%。纯化后其纯度达到95.92%。质谱测定其相对分子质量为918,与理论相对分子质量相符。氨基酸序列测定为NH2/Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu,与设计序列一致。当THF-γ2浓度为300μg.L-1时,小鼠脾细胞产生IL-2的浓度最高,为空白对照的1.9倍。结论在国内首次建立了胸腺体液因子(THF-γ2)从合成、纯化、分析到活性测定的方法,为胸腺体液因子的进一步研究和应用打下了基础。OBJECTIVE To study the synthesis, purification, analysis and activity determination of Thymus Humoral Factor（THF-γ2）. METHODS Thymus Humoral Factor（THF-γ2） was synthesized by Fmoc-SPPS, purified by MPLC, and identified by HPLC, MS and amino acid sequence determination. The biological activity ot THF- γ2 was measured according to its characteristics of promoting IL-2 production in mouse spleen cells. RESULTS The purity of the product was 95.92%, the molecular mass was 918, and the amino acid sequence was NH2/Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu. In the concentration of 300 μg· L^-1, the activity was largest and 1.9 times as much as the control. CONCLUSION The methods of the synthesis, purification, analysis and activity determination of Thymus Humoral Factor（THF-γ2） are established for further research and applications in China.

关 键 词：胸腺体液因子 FMOC IL-2 

分 类 号：Q516[生物学—生物化学]