改良帕金森病大鼠模型的建立及评价  

Establishment and evaluation of an improved rat model of Parkinson disease

作　　者：苏平[1] 李艳华[2] 郑敏[2] 管利东[2] 王韫芳[2] 陆兵勋[1] 裴雪涛[2] 

机构地区：[1]南方医科大学南方医院神经内科,广东省广州市510515 [2]解放军军事医学科学院野战输血研究所,北京市100850

出　　处：《中国临床康复》2006年第14期80-82,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家"八六三"高技术研究发展计划课题基金资助项目(2005AA219010);国家"九七三"重点基础研究发展规划基金资助项目(2001CB509906)~~

摘　　要：目的:建立一种改进的帕金森病大鼠模型制作方法,并对模型进行综合评价。方法:实验于2004-02/2005-06在军事医学科学院干细胞研究中心完成。①选取Wistar大鼠60只,随机分为3组,传统组20只,改良组30只,对照组10只。②传统组向右侧中脑黑质致密部和中脑腹侧被盖区两点各注射6-羟基多巴胺8μg制作传统帕金森病大鼠模型,改良组向中脑腹侧被盖区单点6-羟基多巴胺12μg制作改良帕金森病大鼠模型。对照组向右侧中脑黑质致密部和中脑腹侧被盖区两点各注射含2g/L抗坏血酸的生理盐水4μL。③检测各组大鼠行为学变化及黑质多巴胺能神经元变化。结果:对照组和传统组术后分别有1只和4只动物在1周内死亡,改良组无动物死亡。行为学和免疫组化检测显示:①改良组30只大鼠有22只旋转稳定,平均转数>7r/min,成功率超过70%,与传统组相当。改良组大鼠死亡率低于传统组(20%)和对照组(10%)。②术后传统组和改良组均有部分动物出现震颤、活动迟缓、易激惹、竖毛、尾僵等异常行为。③成功帕金森病模型大鼠中脑黑质注射侧神经元数量明显减少,达90%以上,改良组与传统组比较差异无显著性(P>0.05),与对照组比较注射侧黑质多巴胺能神经元显著减少(P<0.001)。结论:改良帕金森病大鼠模型方法简便实用,动物死亡率低,模型成功率高,可以较快建立稳定的帕金森病大鼠模型。AIM： To study an improved method to establish rat model of Parkinson disease, and evaluate the model comprehensively. METHODS： The experiment was carried out in the Research Center of Stem Cells, Academy of Military Medical Sciences between February 2004 and June 2005. （1） Sixty Wistar rats were randomly divided into traditional group （n=20）, improved group （n=30） and control group （n=10）. （2） The rats were administered with stereotaxic microinjection of 6-hydroxy- dopamine （6-OHDA） into the right substantia nigra compact part and the midbrain ventral tegmental area in the traditional group, and only the midbrain ventral tegmental area in the improved group, and those in the control group were injected with 4 μL saline containing ascorbic acid （2 g/L） into the right substantia nigra compact part and the midbrain ventral tegmental area. （3） The changes of behaviors and the quantity of midbrain nigra dopaminergic neurons were detected. RESULTS： One and 4 rats died within 1 week postoperatively in the control group and traditional group respectively, and there was no death in the improved group. Behavioral test and immunohistochemical detection showed that （1） In the improved group （n=30）, 22 rats rotated stably, with an average 〉7 rounds per minute, and the successful ratio exceeded 70%, which was equivalent to that in the traditional group. The mortality in the improved group was lower than that in the traditional group （20%） and control group （10%）. （2） Postoperatively, part of the rats in the traditional group and improved group had the abnormal behaviors of tremor, retardation, irritability, piloerection and stiffness of tall, etc. （3） The number of dopaminergic neurons in midbrain nigra of the injected side was obviously reduced by more than 90% in the successful rat models of Parkinson disease, and there was no significant difference between the improved group and traditional group （P 〉 0.05）, which were significantly decreased as comp

关 键 词：帕金森病 疾病模型 动物 羟基多巴胺类 黑质 中脑被盖 

分 类 号：R742.5[医药卫生—神经病学与精神病学]