河豚毒素与阿司匹林的协同镇痛作用  

Analgesic effect of tetrodotoxin combined with acetylsalicylic acid

作　　者：符凤英[1] 库宝善[1] 马行[1] 徐英[1] 姚海燕[1] 姜娜[1] 

机构地区：[1]北京大学基础医学院药理学系,北京100083

出　　处：《中国临床康复》2006年第14期100-101,110,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:对比单独应用河豚毒素和单独应用阿司匹林及与两者合用的镇痛效应。方法:实验于2003-10/2005-03在北京大学基础医学院药理学系完成。选用昆明健康小鼠230只。随机分为单用河豚毒素组、单用阿司匹林组和小剂量河豚鼠与阿司匹林联合用药组三大组,共23个亚组,每亚组均为10只。采用小鼠醋酸扭体致痛实验观察合用后是否具有镇痛增强或协同效应。结果:230只大鼠均进入结果分析。①单用河豚毒素组的镇痛作用:河豚毒素组(0.5～4.0)×10-3mg/kg时,小鼠扭体半数抑制量为2.1×10-3mg/kg;当河豚毒素的剂量用到4.0×10-3mg/kg时,小鼠醋酸扭体反应的抑制率为65.2%;②单用阿司匹林的镇痛作用:阿司匹林组(12.5～200)×103mg/kg的镇痛半数抑制量为64.0mg/kg,相应的镇痛抑制百分率为15.8%到93.2%。③小剂量河豚毒素(即0.79×10-3mg/kg,0.39×10-3mg/kg)与阿司匹林联合应用时,镇痛作用明显增强,此时阿司匹林的半数抑制量下降至5.8mg/kg和12.6mg/kg。结论:小剂量河豚毒素和阿司匹林联合应用时能明显提高镇痛疗效。AIM： To compare the analgesic effects of tetrodotoxin （TTX） alone, acetylsalicylic acid （ASA） alone and combination of TTX and ASA. METHODS： The experiment was conducted at the Department of Pharmacology, School of Basic Medical Sciences, Peking University between October 2003 and March 2005.Totally 230 healthy KunMing mice were randomly divided into TTX group, ASA group and combination group, including 23 subgroups with 10 in each subgroup. The reinforced analgesia and synergistic effects of the combination were examined by using the acetic acid-induced writhing test in mice. RESULTS： Totally 230 mice were involved in the result analysis. （1）TTX group： When the mice were injected with （0.5-4.0）×10^-3 mg/kg TTX, the inhibitory dose （ID50） of mice was 2.1×10^-3 mg/kg;When 4.0×10^-3 mg,/kg TTX was used, the inhibitory rate on acetic acid-induced writhing response of mice was 65.2%. （2）ASA group： When injected with （12.5-200）×10^3 mg/kg ASA, the ID50 was 64.0 mg/kg, and the corresponding rates of analgesia inhibition were from 15.8% to 93.2% respectively. （3）The analgesic effects were obviously increased at the small dose of Trx （0.79×10^-3 mg/kg, 0.39×10^-3 mg/kg） plus ASA. The ID50s of ASA were reduced to 12.6 mg/kg and 5.8 mg/kg, respectively. CONCLUSION： The small dose of TTX combined with ASA can markedly increase the analgesic effects.

关 键 词：河豚毒素 阿司匹林 半数抑制浓度(ID50) 

分 类 号：R441.1[医药卫生—诊断学]