心肺复苏后大鼠血清神经元特异性烯醇化酶、S100β蛋白对脑损伤诊断的价值  

The significance of NSE and S100β protein in the diagnosis of early brain injury after cardiopulmonary resuscitation in rats

作　　者：马宇洁[1] 杨兴易[1] 林兆奋[1] 张雷[1] 

机构地区：[1]中国人民解放军第二军医大学附属长征医院急救科,上海200003

出　　处：《中华急诊医学杂志》2006年第4期335-337,共3页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金资助(30500173)

摘　　要：目的观察大鼠心肺复苏后血清神经元特异性烯醇化酶(NSE)和S100β蛋白水平变化,确立CPR后脑损伤具有早期诊断价值的生化指标。方法选择成年雄性Sprague-Dawley大鼠50只,采用窒息合并冰氯化钾停跳液制备大鼠心跳骤停-心肺复苏模型。随机分为5组:对照组即假手术组10只,自主循环恢复(ROSC)后6、12、24、48h组各10只。停跳5min后开始心肺复苏,采用双抗体夹心酶联免疫分析法为对照组及ROSC后6、12、24、48h组进行动态观测血清中NSE、S100β蛋白水平变化。结果在ROSC后6、12、24、48h组的血液中NSE、S100β蛋白水平与对照组比较都有显著升高(P<0.05),但指标升高的时间和幅度并不一致。与对照组比较,血清NSE在24h时点、S100β蛋白在6h时点升高水平差异有极其显著性(P<0.01)。结论血清NSE、S100β蛋白水平可作为评价CPR后脑损伤具有早期诊断价值的指标。Objective To study the changes of neuron specific enolase （NSE） and S100β protein levels in serum after cardiopulmonary resuscitation （CPR） in rats, and and to evaluate their significance on early diagnosis of brain injury. Methods Caridiac arrest model was induced by asphyxiation with ice-cold 0.5 mol KCl in rats, and resuscitation 5 minutes after arrest. Fifty male Sprague Dawley rats were randomly divided into 5 groups ： sham control group （ n = 10） and 4 routine treatment group （experimental group） （n = 10, per group） . The blood samples were taken from rats at 6 , 12 , 24 , 48 hours after restoration of spontaneous circulation （ROSC）. NSE and S100β measured by enzyme-linked immunosorbent assay were compared between each experimental group, serum from sham control animals was also analyzed. Results Both NSE and S100β were significantly increased in serum after ROSC when compared to sham controls （P 〈 0.05 ）. The level of NSE peaked at 24 hours after ROSC （ P 〈 0.01 ）, and the S10013 peaked at 6 hours after ROSC （ P 〈 0.01 ）. Conclusion NSE and S100β could be used to evaluate brain injury after CPR.

关 键 词：心肺复苏 脑损伤 神经元特异性烯醇化酶 S100Β蛋白 

分 类 号：R459.7[医药卫生—急诊医学]