胰液K-ras密码子点突变联合血清CA 199水平与胰腺癌复发关系的研究  

Observation on the Relationship Between K-ras Mutation in Pancreatic Jucice Accompanying by Serum CA 199 Level and Recurrance of Pancreatic Carcinoma

作　　者：刘晓川[1] 孟垂华 刘铁夫[1] 梁桃 

机构地区：[1]哈尔滨医科大学第一临床医学院,哈尔滨150001 [2]哈尔滨锅炉厂医院

出　　处：《临床消化病杂志》2006年第2期95-97,共3页Chinese Journal of Clinical Gastroenterology

摘　　要：目的探讨胰液中K-ras 12密码子点突变联合血清CA199检测与胰腺癌病程的关系。方法测定32例临床及手术证实的胰腺癌患者血清CA199水平,并采用内镜ERCP从胰管收集的胰液标本,聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)检测胰液K-ras 12基因12密码子点突变,分析胰液中K-ras 12密码子点突变及血清CA199联合检测与胰腺癌术后复发的关系。结果(1)胰液中K-ras 12密码子点突变率为56.3%,与肿瘤大小密切相关(P<0.05)。K-ras 12密码子点突变阳性、阴性表达病例3年复发率分别为66.7%和33.3%。(2)高血清CA199水平且K-ras 12密码子点突变阳性病例组3年复发率为69.2%,而低血清CA199且ras阳性病例组3年复发率为20.0%,差异显著(P<0.05)。结论联合胰液中K-ras 12密码子点突变及血清CA199检测可作为判断胰腺癌术后复发的有效指标,多因素分析对胰腺癌术后复发的判断更有价值。Objective To study the relationship between K-ras mutation in pancreatic juice accompanying by serum CA 199 level and recurrence of pancreatic carcinoma. Methods K-ras point mutation was detected using the polymerase chain reaction and restriction fragment-length polymorphism（PCR-RFLP） in pure pancreatic juice （PPJ）, which were collected from the pancreatic duct under endoscopic retrograde clolangiopancreatic tography（ERCP）, And serum CA 199 level were examined in 32 patients with pancreatic carcinoma, The relationships between K-ras, serum CA 199 level and recurrence rate were analyzed, Results （ 1 ） The positivity of K-ras point mutation in PPJ was 56, 30/9 ;The positivity of K-ras point mutation in PPJ correlated with tumors currence rate （P 〈 0.05 ）. （2）The recurrence rate in high-serum CA 199 level group with positive K-ras point mutation in PPJ was 69.2% ,while in hypo-serum CA 199 level was 20. 0% （ P 〈 0.05 ）, Conclusion K-ras mutation in pancreatic juice and serum CA 199 level are effective in dictators for recurrence and prognosis of pancreatic carcinoma, The analysis of muliti-gene is more valuable than that of single.

关 键 词：K—ras 基因 胰液 CA199 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]