反相高效液相色谱法同时测定甘草酸及其酶转化产物  

Simultaneous Determination of Glycyrrhizic Acid and Its Enzymatic Transformation Product by Reversed Phase High Performance Liquid Chromatography

作　　者：卢定强[1] 戴燕[1] 李晖[1] 胡继军[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《精细化工》2006年第4期355-357,共3页Fine Chemicals

基　　金：高等学校博士学科点专项科研基金(20040291004);国家重大基础研究项目(2003CB716000)~~

摘　　要：甘草酸（glycyrrhizic acid，GL）及其衍生物是一类具有重要生理活性的化合物。利用酶转化GL可以生成具有更高生理活性的单葡萄糖醛酸甘草次酸（glycyrrhetyl 3-O-mono-β-D—glucuronide，GAMG）。为了深入研究该酶对GL的生物转化特性，该文建立了一种能同时检测GL及其酶转化产物GAMG的高效液相色谱分析方法。采用Apollo C18分析柱，流动相V（甲醇）：V（水）：V（冰醋酸）=75：25：5，流速1．0mL／min，紫外检测波长254nm。在进样量为0．2—20μg内成良好的线性关系，GL的加样回收率为94．3％-103．2％，相对标准偏差为0．85％-2．53％；GAMG的加样回收率为92．6％-98．7％，相对标准偏差为1．45％-3．25％。方法准确、简捷、耗材低廉。Glycyrrhizic acid（GL） and its derivatives have various important physiological activities. GL can be transformed by enzyme to glycyrrhetyl 3-O-mono-β-D-glucuronide （GAMG） which is more physiologically active than GL. In order to characterise the enzymatic reaction, a method of reversed phase high performance liquid chromatography （RP-HPLC） was developed for the simultaneous determination of GL and GAMG in the enzymatic transformation system. The analysis could be performed with Apollo C18 column using a mobile phase of V（ methanol ） ： V（ water ） ：V（acetic acid ） = 75：25：5 at a flow rate of 1.0 mL/min with UV detection at 254 nm. The linear ranges of GL and GAMG were both between 0.2 -20 μg. The recoveries of GL and GAMG were 94. 3% - 103.2% and 92.6% -98.7% with relative standard deviations of 0.85% -2.53% and 1.45% -3.25% （n =3） respectively. This method is accurate, simple, rapid and cheap for simultaneous determination of GL and GAMG in the enzymatic transformation system.

关 键 词：高效液相色谱法 甘草酸 单葡萄糖醛酸甘草次酸 酶转化 

分 类 号：O657.72[理学—分析化学]