cDNA macroarray for analysis of gene expression profiles in prostate cancer  

作　　者：ZHONG Wei-de HE Hui-chan BI Xue-cheng OU Ru-biao JIANG Shao-ai LIU Liang-shi 

机构地区：[1]Department of Urology, Guangzhou First Municipal People＇s Hospital, Guangzhou Medical College, Guangzhou 510180, China [2]College of Life Science, Sun Yat-sen University, Guangzhou 510180, China

出　　处：《Chinese Medical Journal》2006年第7期570-573,共4页中华医学杂志（英文版）

摘　　要：Background Early diagnosis and timely treatment are important for improving therapeutic efficiency of prostate cancer. DNA array is a new bio-technology for disease diagnosis. This study was conducted to diagnose prostate cancer with cDNA macroarray and analysis gene expression profiles of some selective genes in prostate cancer. Methods Total RNA was isolated from patients with prostate cancer and from normal people, and poly（A） RNA was further purified. Then it was analyzed for differentially expressed genes in prostate cancer and normal prostate by cDNA macroarray system. Results There were different expressions in the nine prostate-associated specific genes in prostate cancer as compared with normal prostate, in which, 7 were significantly upregulated and 2 were down-regulated. Conclusion As a diagnostic approach at molecular level, the cDNA macroarray is an effectively diagnostic method for orostate cancer.Background Early diagnosis and timely treatment are important for improving therapeutic efficiency of prostate cancer. DNA array is a new bio-technology for disease diagnosis. This study was conducted to diagnose prostate cancer with cDNA macroarray and analysis gene expression profiles of some selective genes in prostate cancer. Methods Total RNA was isolated from patients with prostate cancer and from normal people, and poly（A） RNA was further purified. Then it was analyzed for differentially expressed genes in prostate cancer and normal prostate by cDNA macroarray system. Results There were different expressions in the nine prostate-associated specific genes in prostate cancer as compared with normal prostate, in which, 7 were significantly upregulated and 2 were down-regulated. Conclusion As a diagnostic approach at molecular level, the cDNA macroarray is an effectively diagnostic method for orostate cancer.

关 键 词：prostate neoplasm cDNA macroarray gene expression profiles 

分 类 号：R737.25[医药卫生—肿瘤]