胆系恶性肿瘤p16,PTEN和nm23基因的表达及其临床意义  

Study on clinical significance of expression of p16,nm23 and PTEN gene in malignant tumors of biliary system

作　　者：崔平[1] 普苹[2] 赵晓霞[2] 吴韬[1] 杨世昆[1] 胡筑培[1] 

机构地区：[1]昆明医学院第一附属医院肝胆外科,云南昆明650031 [2]昆明医学院第一附属医院病理检验科,云南昆明650031

出　　处：《中国普通外科杂志》2006年第3期195-197,共3页China Journal of General Surgery

摘　　要：目的探讨胆系恶性肿瘤组织中p 1 6,nm 2 3和PTEN基因蛋白表达的临床意义。方法利用SP免疫组化方法检测6 3例胆系恶性肿瘤(胆管腺癌3 3例,胆囊腺癌3 0例)和2 0例胆管良性病变组织中p 1 6,PTEN和nm 2 3基因蛋白的阳性表达情况,结合临床病理资料进行分析。结果p 1 6,nm 2 3及PTEN 3种基因蛋白在胆道腺癌组织中呈低-中度阳性表达,其阳性表达率分别为36.5%,3 8.1%,2 3.8%,明显低于胆管良性病变组,差异有统计学意义(P<0.0 5)。PTEN在高分化胆管腺癌与胆囊腺癌表达有明显差异(P<0.0 5),而p 1 6和nm 2 3差异不明显。结论胆系恶性肿瘤中存在上述3种基因变异或缺失。PTEN和p 1 6基因变异对细胞周期均失去调控,从而促进肿瘤生长,PTEN和nm 2 3基因的变异降低了对胆系肿瘤浸润转移的制约。Objective To study the clinical significance of the gene protein expression of p16, nm23 and PTEN in bile duct carcinoma. Methods The expression of p16, PTEN and nm23 gene protein in tissues of 63 cases of malignant tumors of biliary system （ 33 cases of bile duct carcinoma, 30 cases of carcinoma of gallbladder ） and 20 cases of benign bile duct lesion were studied by SP immunohistochemical technique and analyzed together with clinicopathologic data. Results The positive expression of p 16, PTEN and nm23 in bile duct carcinoma tissue was low to medium, their expression rate was 36. 5 % , 38. 1% and 23. 8% , respectively, and was significantly lower than that of bile duct benign lesions （P 〈 0. 05 ）. In the well-differentrated bile duct carcinoma and gallbladder carcinoma group, the expression of PTEN was significantly different（ P 〈 0. 05 ）, but p16, PTEN and nm23 expression was not significantly different. Conclusions Mutation or deletion of the above 3 genes is present in malignant tumors of biliary system. PTEN and p 16 gene mutation can loss control of the cell cycle and thus promote tumor development. PTEN and nm23 gene mutation can lower the controlability of invasiveness and metastasis of tumors of biliary system.

关 键 词：胆道肿瘤 病理学 基因表达 癌基因蛋白类 

分 类 号：R735.8[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]