局灶性脑缺血后老年大鼠室管膜下区和颗粒下层细胞增殖与分化的实验研究  

PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS IN THE SUBVENTRICULAR ZONE AND SUBGRANULAR ZONE IN OLD RATS AFTER FOCAL CEREBRAL ISCHEMIA

作　　者：刘俊华[1] 晋光荣[1] 李云涛[2] 徐汉荣[1] 杨鹏[1] 

机构地区：[1]东南大学医学院解剖学与组织胚胎学系,南京210009 [2]南京医科大学第二附属医院神经内科,南京210003

出　　处：《解剖学报》2006年第2期140-144,共5页Acta Anatomica Sinica

基　　金：江苏省自然科学基金资助项目(BK2003059)

摘　　要：目的观察老年大鼠局灶性脑缺血后室管膜下区(SVZ)和颗粒下层(SGZ)神经干细胞的增殖与分化。方法取老年大鼠制作大脑中动脉梗塞模型。用5-溴脱氧尿核苷(BrdU)脉冲标记结合免疫组织化学单标记技术,观察正常组、假手术组、脑缺血后3、71、4、212、8 d组SVZ和SGZ区BrdU阳性细胞的变化;用BrdU累积标记结合免疫组织化学双标技术,观察脑缺血14 d后SVZ和SGZ区BrdU/NeuN和BrdU/GFAP双标阳性细胞的数量。结果在正常组、假手术组及各脑缺血组大鼠的双侧SVZ和SGZ均可观察到BrdU阳性细胞。与正常组和假手术组相比,脑缺血后SVZ和SGZ区BrdU阳性细胞明显增加。缺血组SVZ区BrdU阳性细胞在脑缺血后7 d时达到高峰,28 d时仍高于正常水平;SGZ区BrdU阳性细胞在脑缺血后14 d时达到高峰,28 d时仍高于正常水平。通过BrdU累积标记和免疫组织化学双标发现:脑缺血14 d后,老年大鼠SVZ区有部分细胞显示BrdU/NeuN(0.98%)或BrdU/GFAP(12.56%)双标阳性,而SGZ区未见双标细胞。结论局灶性脑缺血可激活老年大鼠室管膜下区和颗粒下层的神经干细胞明显增殖,并且室管膜下区有部分增殖细胞可分化为神经元或神经胶质。Objective The proliferation and differentiation of neural stem cells in the subventricular zone（SVZ） and subgranular zone（SGZ） have been observed in normal old rats or treated by middle cerebral artery occluded（MCAO） and sham operation respectively. Methods Old rats were used to make models of middle cerebral artery occluded（ MCAO）. By BrdU pulse labeling and immunohistochemical single staining, the change of the number of BrdU positive cells was observed in SVZ and SGZ of normal rats or cerebral ischemia rats after MCAO 3d,7d, 14d,21d,28d and sham operation respectively. By BrdU accumulating label and immunohistochemical double staining, BrdU/NeuN and BrdU/GFAP positive cells were determined in SVZ and SGZ of aged rats after MCAO 14d. Results BrdU positive cells were found in bilateral SVZ and SGZ of all groups above mentioned. Compared with normal and sham groups, BrdU positive cells of SVZ and SGZ in cerebral ischemia rats were strikingly increased. BrdU positive cells reached the peaks in SVZ at 7d and in SGZ at 14d after MCAO. At 14d after MCAO, BrdU/NeuN（0.98% ） or BrdU/GFAP（ 12.56% ） double labled cells were observed in SVZ, but no double labled cell was observed in SGZ. Conclusion The focal cerebral ischemia markedly stimulated proliferation of neural stem cells in SVZ and SGZ in old rats, and some proliferative cells in SVZ can differentiate into neurons or glial cells.

关 键 词：局灶性脑缺血 室管膜下区 颗粒下层 免疫组织化学 BRDU标记 老年大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]