杂色曲霉素单次灌胃后小鼠第四脑室外侧隐窝超微结构的变化  

ULTRASTRUCTURAL CHANGES OF CEREBRAL TISSUE IN THE LATERAL RECESS OF THE FOURTH VENTRICLE IN BALB/c MICE AFTER SINGLE ORAL ADMINISTRATION OF STERIGMATOCYSTIN

作　　者：郝庆卯[1] 张祥宏[1] 邢凌霄[1] 严霞[1] 王俊灵[1] 马常升[1] 刘贵生[1] 王凤荣[1] 

机构地区：[1]河北医科大学基础医学研究所病理研究室,石家庄050017

出　　处：《解剖学报》2006年第2期149-152,共4页Acta Anatomica Sinica

基　　金：教育部科学技术研究重点项目(205021);河北省自然科学基金项目(301350)资助

摘　　要：目的观察杂色曲霉素(ST)对外侧隐窝处室管膜细胞、神经元、神经纤维和血管内皮细胞的影响。方法BALB/c小鼠,ST单次灌胃,分别于灌胃后1、2、4、81、6 h处死动物,用透射电镜观察外侧隐窝室管膜细胞、神经元、神经纤维和血管内皮细胞的结构变化。结果ST灌胃后1 h血管内皮细胞吞饮囊泡开始增多,2 h组室管膜细胞的顶浆分泌增多,部分神经髓鞘变性;4 h和8 h组神经纤维广泛变性,神经元线粒体变性、核切迹和空泡出现;16 h组神经元胞浆内线粒体变性更加明显,并出现脂褐素。结论单次灌胃后,ST即可引起外侧隐窝处室管膜细胞、神经纤维、神经元和内皮细胞的改变,但ST主要是对神经纤维和神经元的损伤。Objective To investigate the effects of sterigmatocystin（ST） on the ependymal cells, neurons and endothelial cells of blood vessel of the lateral recess of the fourth ventricle in BALB/c mice in vivo after single oral administration. Methods BALB/c mice were treated intragastrically by gavage with ST 3000 μm/kg. The mice were killed 1 hour, 2 hours, 4 hours, 8 hours, 16 hours after oral administration respectively for observing ependymal cells, neurons and endothelial cells of blood vessel of the lateral recess with transmission electron microscopy （TEM）. Results After oral administration of ST pinocytosis vesicles began to increase in endothelial cells of blood vessel in 1 hour, apocrine of ependymal cells began to increase and myelin sheath degeneration appeared in 2 hours, extensive myelin sheath degeneration and mitochondrion degeneration and incisure and vacuoles in nucleus of neuron were found in 4 and 8 hours. Lipofuscin was found in cytoplasm of neuron 16 hour. Conclusion Oral ST exposure in mice could cause myelin sheath degeneration and damaged changes of neurons.

关 键 词：外侧隐窝 室管膜细胞 神经元 血管内皮细胞 杂色曲霉素 电子显微镜术 小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]