伸展细胞原代培养与生物学特性研究  

THE ESTABLISHMENT OF MODEL FOR PRIMARY CULTURED TANYCYTES

作　　者：周馨[1] 高秀来[1] 马育平[1] 陈亚亮[1] 李莉[1] 刘霞[1] 景朋[1] 陆涛[1] 

机构地区：[1]首都医科大学解剖学教研室,北京100069

出　　处：《解剖学报》2006年第2期181-183,139,共4页Acta Anatomica Sinica

基　　金：北京市教委基金资助项目(00KJ104)

摘　　要：目的体内定位生后7d大鼠的伸展细胞(TAs),并将其分离进行原代培养,为进一步移植提供指导。方法免疫组织化学的方法,利用波形蛋白(VIM)和胶质细胞纤维酸性蛋白(GFAP)两种标志物,体内定位生后7 dWistar大鼠的TAs;NADPH-d组织化学方法,定性检验其一氧化氮合酶(NOS)的表达;细胞培养技术,建立TAs体外培养的细胞模型并对其进行鉴定。结果原代培养的TAs表达VIM、GFAP和NOS与体内相同。结论建立了体外研究TAs的细胞模型,为其进一步作为移植细胞提供了可能性。Objective Tanycytes（TAs） is a specialized eppendymal glia that locates mostly in the ventral lateral wall of the ventricle Ⅲ and median eminence（ME）. Due to its peculiar location and directly exposure to the cerebrospinal fluid, blood, neuroendocrine hormones and neurons, tanycytes play an important role in the brain barrier system, brain-CSF neurohumoral circuit and immune-neuroendocrine network. They maintain immature characters during the adulthood and have naturally conducted the neuroregeneration process in the adult hypothalamus of mammal animals. This research was designed to identify tanycytes（TAs） in postnatal 7 days of Wistar rats and then establish the cell model of TAs for further study. Methods Tanycytes of postnatal 7 days of Wistar rats were identified by the immunohistochemical technology using glial fibrillary acidic protein （GFAP） and vimentin （VIM） ,which are the intermediate filament markers of glia cells. The qualitative analysis was performed by the NADPH-d stain of nitric oxide sythenase（NOS）. Primary TAs model was established by the cell culture technique and identified by the same methods as in vivo. Results Primary cultured TAs expressed VIM, GFAP and NOS which was identical with those in vivo. Conclusion Cell model of TAs from postnatal 7days of Wistar rats in vitro has been established and TAs could be used as a proper substrate for transplanting.

关 键 词：伸展细胞 正中隆起 一氧化氮 神经再生 细胞培养 大鼠 

分 类 号：R322.8-3[医药卫生—人体解剖和组织胚胎学]