Association of the HLA-DRB1*0701 allele with perinuclear anti-neutrophil cytoplasmatic antibodies in Mexican patients with severe ulcerative colitis  

作　　者：Jesus K Yamamoto-Furusho Luis Uscanga-Domínguez Alondra Lopez-Martinez Julio Granados 

机构地区：[1]Department of Gastroenterology Institute Nacional de Ciencias Médicas y Nutrición Salvador Zubirán.México,D F,México [2]Department of Immunology Institute Nacional de Ciencias Médicas y Nutrición Salvador Zubirán.México,D F,México

出　　处：《World Journal of Gastroenterology》2006年第10期1617-1620,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by Consejo Nacional de Ciencia y Tecnologia CONACYT,NO. 153237

摘　　要：AIM： To determine the association between the HLADRB1 alleles and perinuclear anti-neutrophil cytoplasmatic antibodies （p-ANCA） positive in Mexican patients with ulcerative colitis （UC）. METHODS： Ninety Mexican mestizo patients （45 females） with UC, confirmed by biopsy, were studied. High resolution HLA typing was performed by PCR-SSO reverse dot blot and PCR-SSP. Molecular typing techniques were applied to define HLA-DRB1 alleles. Enzyme-linked immunosorbent assay and immunofluorescence techniques were used to detect p-ANCA. RESULTS： Forty-eight （53%） UC patients were positive for p-ANCA by ELISA and IF. We found that p-ANCA- positive UC patients had a significantly increased frequency of HLA-DR7 compared with p-ANCA-negative controls （22% vs 5.1%; pC=0.02, OR=5.2, CI 95%： 1.06-37.82）. Disease activity was scored as severe in 20 patients, moderate in 8, mild in 14 and no activity in the remaining 38 patients according to the Truelove and Witts criteria. Subgroup analysis showed a significantly increased frequency of the HLA-DRB1＊07 allele in 15 of 20 UC patients with severe activity of UC and p-ANCA positivity [100% vs 0%; pC=0.0000001; OR=35]. No significant differences were found between p-ANCA positive patients, HLA-DR alleles and other clinical features such as extraintestinal manifestations, proctocolectomy and extension.CONCLUSION： The HLA-DRB1＊07 is associated with p-ANCA positive UC Mexican patients.瞄准：决定在 HLA-DRB1 等位基因和仙子之间的协会原子反嗜中性的 cytoplasmatic 抗体(p-ANCA ) 在有 ulcerative (UC ) 的墨西哥病人积极。方法：有 UC 的 90 个墨西哥混血儿病人(45 女性) ，由活体检视证实了，被学习。打字的高决定 HLA 被 PCR-SSO 反向的点污点和 PCR-SSP 执行。分子的打字技术被使用定义 HLA-DRB1 等位基因。连接酶的免疫吸着剂试金和免疫荧光技术被用来检测 p-ANCA。结果：48 (53%) UC 病人为由 ELISA 的 p-ANCA 是积极的并且如果。我们发现 p-ANCA-positive UC 病人与 p-ANCA-negative 控制相比有 HLA-DR7 的显著地增加的频率(22% 对 5.1% ；pC=0.02， OR=5.2， CI 95% ：1.06-37.82 ) 。疾病活动在 20 个病人，作为严重被获得在 8 中等，在 14 并且不温和在留下的活动 38 个病人根据纯爱和 Witts 标准。亚群分析与 UC 和 p-ANCA 确实的严重活动在 20 个 UC 病人中的 15 个显示出 HLA-DRB1 *07 等位基因的显著地增加的频率[100% 对 0% ；pC=0.0000001；OR=35 ] 。没有有效差量在 p-ANCA 之间被发现象额外的肠的表明， proctocolectomy 和延期那样的积极病人， HLA 医生等位基因和另外的临床的特征。结论：HLA-DRB1 *07 与 p-ANCA 被联系积极 UC 墨西哥病人。

关 键 词：HLA-DR P-ANCA Ulcerative colitis MEXICANS 

分 类 号：R574.62[医药卫生—消化系统]