p53基因BstUⅠ位点多态性与非小细胞肺癌易感性及放射敏感性的相关性研究  

Association of polymorphism of codon 72 in p53 gene with susceptibility and radiosensitivity of non-small cell lung cancer in Chinese population

作　　者：李健强[1] 邵国光[1] 刘琳琳[2] 郑永晨[3] 

机构地区：[1]吉林大学第二临床学院胸外科,长春130041 [2]吉林大学第二临床学院放疗科,长春130041 [3]吉林大学第二临床学院中心实验室,长春130041

出　　处：《中国肺癌杂志》2006年第2期173-176,共4页Chinese Journal of Lung Cancer

基　　金：吉林省科技厅社会发展基金(No.92226000)资助~~

摘　　要：背景与目的肺癌是世界上发病率和死亡率增长较快的恶性肿瘤。近几年来随着分子生物学的蓬勃发展,人们对疾病的认识达到了基因水平。通过分子生物学方法发现某一基因与肺癌之间的关联性并加以利用,为早期诊断及治疗肺癌提供了一个新的思路。本研究拟探讨p53肿瘤抑制基因BstUⅠ位点多态性与非小细胞肺癌(NSCLC)易感性及放射敏感性的相关性。方法采用PCRRFLP方法检测50例NSCLC患者及50例健康对照p53基因序列中的BstUⅠ位点单核苷酸多态性(SNP)。并采用病例对照方法分析p53基因BstUⅠ位点的多态性与肺癌的易感性,以及NSCLC患者p53基因BstUⅠ位点的多态性与放疗疗效的相关性。结果不同p53基因BstUⅠ基因型的NSCLC患者对放疗的有效率不尽相同。杂合子(A1/A2)基因型的患者对放疗的有效率低,仅为25.0%,而纯合子(A1/A1,A2/A2)基因型的患者对放疗的有效率较高,分别为71.4%和70.0%,三者之间的差异有显著性(χ2=9.2,P<0.05)。肺癌组的A1/A1、A1/A2及A2/A2基因型频率分别为28.0%、32.0%及40.0%,对照组为32.0%、42.0%及26.0%,但两组间纯合子的频率差异并无统计学意义(P均>0.05),并且未观察到p53基因BstUⅠ的多态性与NSCLC易感性有密切的相关关系。结论p53基因BstUⅠ位点多态性可能与NSCLC发生无关,但NSCLC患者的p53基因BstUⅠSNP可能与放射敏感性有关。Background and objective Lung cancer is one of the most common cancers worldwide and its mortality rate has increased year after year. Molecular biology has contributed to make people＇s understanding to the disease at the gene level. A new idea will be given to the early diagnosis and treatment for lung cancer with the method of molecular biology which can be used to find the association between some genes and lung cancer. The aim of this study is to evaluate the relationship between polymorphism of codon 72 （BstUⅠ single nucleotide polymorphism, BstUⅠ SNP） in p53 gene and susceptibility and radiosensitivity of non-small cell lung cancer （NSCLC） in Chinese population. Methods The BstUⅠ single nucleotide polymorphic sites at condon 72 in exon 4 of p53 gene in 50 patients with NSCLC as well as 50 healthy controls were inspected by polymerase chain reaction-restricted fragment length polymorphism assay and the relationship between BstUⅠ SNP and susceptibility and radiosensitivity of NSCLC was analysed by case-control test. Results The allelic distribution of the three genotypes （A1/A1, A1/A2, A2/A2） in healthy controls was 32. 0%, 42.0% and 26.0% respectively, which differed slightly from that of lung cancer patients, which was 28.0%, 32.0% and 40. 0%. These allelic and genotype differences between control and lung cancer groups （A1/A1 OR=0.83, 95% CI 0. 36-1.27 and A2/A2 OR=1. 0, 95% CI 0.82-4. 42） were insignificant. The patients with A1/A1 or A2/A2 genotype were sensitive to radiotherapy and the patients with A1/A2 were not sensitive to radiotherapy （χ^2 =9.2, P〈0.05）, the effective rate to radiotherapy were 71.4%, 70. 0% and 25.0% respectively. Conclusion There is no significant relationship between the BstUⅠ SNP in p53 and susceptibility in NSCLC. The p53 BstUⅠ SNP is closely associated with radiosensitivity of NSCLC in the Northern Chinese population.

关 键 词：单核苷酸多态性 非小细胞肺癌 P53基因 放射敏感性 易感性 

分 类 号：R734.2[医药卫生—肿瘤]