nm23-H1质粒和顺铂白蛋白联合治疗裸鼠移植瘤  

Effect on Xenograft of Nude Mouse by Combination Therapy of nm23-H1 and Protein-cisplatin

作　　者：郅克谦[1] 陈伟辉[2] 温玉明[3] 

机构地区：[1]西安交通大学口腔医院颌面外科,陕西西安710004 [2]福建医科大学附属协和医院颌面外科,福建福州350001 [3]四川大学华西口腔医学院口腔颌面外科学教研室,四川成都610041

出　　处：《华西口腔医学杂志》2006年第2期170-172,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(39870746);福建省自然科学基金重点资助项目(C0220002)

摘　　要：目的研究nm23-H1质粒和顺铂白蛋白联合应用对裸鼠移植瘤的影响。方法将15只BALB/C雌性裸鼠随机等量分为3组,即对照组、顺铂白蛋白组和顺铂白蛋白+nm23-H1质粒组。每只裸鼠均皮下注射浓度为每毫升 3．1×106个的舌鳞癌Tca8113细胞,2周后顺铂白蛋白+nm23-H1质粒组移植瘤瘤体内注射质粒-脂质体复合物,3d后顺铂白蛋白+nm23-H1质粒组和顺铂白蛋白组瘤体内注射顺铂白蛋白。观察裸鼠的重量、移植瘤体积和瘤体重量的变化。结果对照组裸鼠重量最轻。nm23-H1质粒和顺铂白蛋白联合治疗组肿瘤体积最小,瘤体重量最轻。结论 nm23-H1基因治疗和顺铂白蛋白联合可以明显抑制裸鼠移植瘤的生长。Objective To study the effect of protein-eisplatin and nm23-H1 therapy on the tumor of nude mouse.Methods The 15 BALB/C female mice were divided into three groups, control group, protein-cisplatin group and protein-cisplatin＋nm23-H1 plasmid group. Tca8113 were injected into the mice subcutaneously with the concentration of 3.1×10^6 cells/mL. After two weeks, the mixture of lipofectin and nm23-H1 was injected around xenograft of nude mouse. After three days, the protein-cisplatin was injected around xenograft. The weight of mouse, the volume and the weight of xenograft were measured. Results The weight of mouse was lightest in control group. The volume and weight of the transplanted tumor were lightest in nm23-H1 ＋protein-cisplatin group. Conclusion The combination therapy of nm23-H1 and protein-cisplatin can effectively inhibites the growth of xenograft in nude mouse.

关 键 词：NM23-H1 顺铂 移植瘤 

分 类 号：R73-3[医药卫生—肿瘤]