Ⅳ型胶原在贲门癌自体荧光诊断中的实验研究  

Significance of the collagen type Ⅳ in the auto-fluorescence diagnosis of cardiac cancer

作　　者：李威[1] 陈胜林[1] 曾永明[1] 陈君填[1] 郭毓文[1] 李欣欣[1] 

机构地区：[1]汕头大学医学院第一附属医院外二科,广东汕头515041

出　　处：《中国医师杂志》2006年第3期314-316,共3页Journal of Chinese Physician

基　　金：2004年广东省医学科学技术研究基金资助项目(A2004418)

摘　　要：目的比较胃底贲门正常组织和癌组织中Ⅲ、Ⅳ型胶原蛋白分布差异,分析胃底贲门组织癌变后Ⅲ、Ⅳ型胶原蛋白分布的改变对组织的自体荧光产生的影响。方法采用免疫组化技术,半定量计分分析Ⅲ、Ⅳ型胶原在16例贲门癌病人正常和癌组织中的分布差异。结果正常组织的基底膜呈现很强的Ⅳ型胶原抗体阳性反应,在基底膜形成较粗的环形染色带;Ⅲ型胶原抗体阳性反应在细胞外间质呈连续的细丝纤维样。癌组织Ⅳ型胶原抗体反应在癌巢周边为阴性,或仅在残存的基底膜有片断的阳性反应;而Ⅲ型胶原在癌间质中的抗体阳性反应较正常组织变化不明显,或还稍有增强;Ⅲ型和Ⅳ型在正常组织和癌组织中的分布差异有统计学意义(P<0.001)。结论贲门癌组织中细胞外基质内Ⅳ型胶原比正常组织显著减少,而Ⅲ型胶原减少不明显;Ⅳ型胶原可能是胃底贲门黏膜细胞外基质自体荧光物质的主要来源。细胞外基质中Ⅳ型胶原的降解可能是癌组织自体荧光减弱的主要原因之一。Objective To compare the distribution of collagen type Ⅲ and Ⅳ in mucosa of normal and carcinoma cardiac tissues and to analyze the influence of changed collagen on auto-fluorescence after canceration. Methods Using immunohistochemical technique with monoclonal anti-collagen type Ⅲ and Ⅳ, the difference of distribution in extracellular matrix of two types collagen between normal and carcinoma tissues from 16 patients were measured with semi-quantitative score methods. Results In normal mucosa, the epithelial basement membrane showed an intense positive reaction for monoclonal anti-collagen Ⅳ and an intensively stained ring band was formed around the grand basement membrane. The collagen type Ⅲ formed ring-like structures apparently representative of fibriform collagen immediately adjacent to the epithelial basement membrane. In carcinomatous mucosa, immunoreaction of the carinomatous cell nest was negative, and only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ. The change of collagen type Ⅲ was not apparent and the positive reaction in carcinomatous stroma was more intensity than in normal stroma. There was significant difference in distribution of collagen type Ⅲ and Ⅳ between the normal and carcinomatous tissues （P 〈 0.001）. Conclusion After canceration of cardiac tissue, the expression of collagen type Ⅳ in basement membrane and stroma is decreased significantly, but that of collagen type Ⅲ remains unchanged. The main collagen located in basement membrane is collagen type Ⅳ and it is the main source of laser-induced auto-fluorescence in cardiac cancer basement membrane. The degradation of collagen type Ⅳ in basement membrane and stroma is the main causes of reduced intensity of laser-induced auto-fluorescence in carcinomatous tissues.

关 键 词：胃肿瘤 贲门 胶原Ⅳ型 胶原Ⅲ型 荧光免疫测定 

分 类 号：R735.2[医药卫生—肿瘤]