增强MRI在大鼠急性脑缺血再灌注血脑屏障损伤中的研究  

Contrast-enhanced MRI in diagnosing damage of blood-brain barrier during reperfusion in rats suffering acute cerebral ischemia

作　　者：黄海东[1] 徐凯[1] 孔凡彬[1] 

机构地区：[1]徐州医学院附属徐州市第一人民医院CT室,221006

出　　处：《中国CT和MRI杂志》2006年第2期43-45,共3页Chinese Journal of CT and MRI

摘　　要：目的通过对大鼠局灶脑缺血再灌注的研究评价增强MRI在判定血脑屏障损伤中的作用。方法采用线栓法制备大鼠右大脑中动脉缺血(MCAO)再灌注模型,雄性SD大白鼠24只(体重280～350g)随机分为2大组:对照组(A组)和缺血组(B组),每组再分为缺血2h组(A1、B1)及缺血6组(A2、B2),再灌注后2h行增强磁共振检查,测得兴趣区信号强度,结合脑组织中伊文氏蓝(EB)的含量进行比较分析。结果A1、A2组再灌注后均无强化,未见伊文氏蓝染色。缺血组与对照组增强MRI信号强度比有差异(P<0.05),B1、B2组之间信号强度比有差异(P<0.05),缺血组右侧大脑半球EB含量与对照组相比有差异(<0.05),B1、B2组之间右侧大脑半球EB含量相比有差异(P<0.05)。结论早期增强MRI检查能灵敏反映血脑屏障的破坏情况,预测继发出血的可能性。Objective To probe role of contrast-enhanced MRI in assessing damage of blood-brain barrier （BBB） during reperfusion after occlusion of right middle cerebral artery in rats. Methods All 24 SD rats were divided into two groups, group A （n=12） was sham-operated as control, group B （n=12） experienced occlusion and reperfusion of the right middle cerebral artery with thread, then each group was divided into two subgroups： group A1、B1 （MCAO 2 hours） and A2、B2 （MCAO 6 hours）. Post-contrast T1WI were performed 2 hours after reperfusion, signal intensity （SI） in the region of interest （ROI） was calculated and the content of Evans blue in the brain tissue was measured. Compare data collected. Results In group A1、A2, no enhancement of parenchyma were found, Evans blue was negative, signal intensity between ischemic group and control group was significantly different （P〈0.05）, and between group B1 and B2 was also significantly （P〈0.05）. The content of Evans blue in the right hemisphere between group A and B was significantly different ischemic and control group （P〈0.05）, was also significantly between B1 and B2 group （P〈0.05）. Conclusion Early contrast-enhanced MRI is sensitive to reflect damage of blood-brain barrier, helping to predict ensuing hemorrhage.

关 键 词：脑缺血 磁共振成像 血脑屏障 伊文氏蓝 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]