应用组织芯片技术分析卵巢癌组织中Bit1基因的表达  

Tissue microarray assay of Bit1 expression in ovarian cancer.

作　　者：滑玮[1] 辛晓燕[1] 张伟[2] 吕海鹏 

机构地区：[1]第四军医大学西京医院妇产科 [2]第四军医大学基础部生物化学与分子生物学教研室 [3]第四军医大学口腔医院牙体牙髓病科,西安710032

出　　处：《现代妇产科进展》2006年第3期181-183,F0003,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:研究B it1基因在正常卵巢组织和不同病理分级的卵巢癌组织中的表达。方法:应用组织芯片技术,通过免疫组织化学方法检测82例卵巢癌标本和78例正常卵巢组织标本中B it1基因的表达。结果:B it1基因在卵巢癌和正常卵巢组织中的弱阳性及阳性表达率分别为100%和33.3%,差异有统计学意义(P<0.01);B it1基因在不同病理分级的卵巢癌组织中的阳性表达率分别为:Ⅰ级,53.1%;Ⅱ级,28.1%;Ⅲ级,7.1%。统计学分析表明,B it1基因在Ⅰ级与Ⅱ级或Ⅲ级的卵巢癌组织中的表达存在显著的统计学差异(P=0.042,0.003),而在Ⅱ级和Ⅲ级中的表达则无统计学差异(P=0.143)。结论:B it1基因在正常卵巢组织中的表达明显低于卵巢癌组织,而且卵巢癌组织中B it1基因的表达与病理分级密切相关。Objective：To study the expression of Bit1 in ovarian cancer and normal ovarian tissues. Metheds：The expression of Bit1 in 82 ovarian cancer samples was analyzed by tissue microarray technology and immunohistochemistry,compared with 78 normal ovarian tissue samples. Resuits ：The positive expression of Bit1 was observed in 100% of ovarian cancer and 33.3% of normal ovarian tissues, respectively. There was significant statistical difference between the expression of Bit1 in two kinds of tissues （P 〈0.01 ）. The expression of Bit1 was observed in 53.1% ,28. 1% and 7. 1% of grade I,grade Hand m ovarian cancer tissues,respectively. Statistical analysis revealed that there was significant difference between the expression of Bit1 in stage Iand grade Ⅱor m ovarian cancer tissues（P =0. 042,0. 003）,but there was no significant difference between the expression of Bit1 in stage Ⅱ and m ovarian cancer tissues（P =0.143 ）. Conclusion：Bit1 is expressed differently in ovarian cancer and normal ovarian tissues,the expression of Bit1 in ovarian cancer has significant relationship with histological grade.

关 键 词：基因Bitl 组织芯片 卵巢肿瘤 基因 

分 类 号：R737.3[医药卫生—肿瘤]