寡聚体透明质酸逆转A549/DDP细胞耐药作用的研究  

A Study of the Effects of Reversing Drug Resistant of Hyaluronate Oligomerson A549/DDP

作　　者：王宝中[1] 司海运[1] 王丽萍[1] 王保君 杨宁[1] 

机构地区：[1]聊城市人民医院,山东聊城252000 [2]临西县第二人民医院,河北临西054900

出　　处：《肿瘤基础与临床》2006年第2期118-119,共2页journal of basic and clinical oncology

摘　　要：目的研究寡聚体透明质酸逆转A549/DDP细胞耐药的作用。方法MTT法研究寡聚体透明质酸对A549/DDP耐药逆转作用,流式细胞术测定细胞表面耐药相关蛋白P-gp、MRP的表达及凋亡调控蛋白P53、bcl-2的表达。结果寡聚体透明质酸可提高A549/DDP对化疗药物的敏感性,使P-gp、MRP表达下降,P53表达增高。结论寡聚体透明质酸有部分逆转A549/DDP耐药及诱导凋亡的作用。Objective To study the effects oi hyaluronate oligomers on reversing drug-resistance and apoptosis of A549/DDP. Methods In application of tetrazolium dye assay to study the reversal effects of hyaluronate oligomers on A549/DDP. MDR related protein P-gp, MRP,apoptosis adjusted protein P53 and bcl-2 were detected by flow cytometry（FCM）.Results Hyaluronate oligomers could enhance the sensitivity of A549/DDP on DDP and expression of P53,and reduce the expression of P-gp and MRP.Conclusions Hyaluronate oligomers could reverse muhidrug resistance in A549/DDP.

关 键 词：寡聚体透明质酸 逆转耐药 诱导凋亡 A549/DDP 

分 类 号：R730.53[医药卫生—肿瘤]