质型多角体病毒在家蚕体内入侵与复制研究(英文)  

Penetration and Replication of Bombyx mori Cytoplasmic Polyhedrosis Virus in vivo

作　　者：孙京臣[1] 陈冬妮 杨艺峰[2] 张勤奋[2] 谭佩婵[1] 徐兴耀[1] 邱宝利[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]中山大学生命科学院生物防治国家重点实验室

出　　处：《中山大学学报（自然科学版）》2006年第2期78-82,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(5300839);华南农业大学校长基金资助项目(2005K004)

摘　　要：采用在家蚕活体内研究家蚕质型多角体病毒(BmCPV)的入侵增殖复制过程,探讨BmCPV的入侵过程和增殖复制机制。实验用健康的三龄起蚕经口接种BmCPV后不同时间取中肠后部固定,制作电镜样品,在透射电子显微镜下观察,结果发现BmCPV病毒被食下1.5 h后,在中肠上皮组织圆筒形细胞微绒毛之间有病毒粒子存在,6 h后病毒进入微绒毛内,并向细胞质移动,9 h后观察到圆筒形细胞质中有病毒粒子。作者认为病毒入侵是以整个病毒粒子穿越中肠围食膜,吸附并进入微绒毛,感染圆筒形细胞,与前人病毒入侵只是髓核进入细胞,而病毒衣壳仍留在细胞外的推测不同。随后病毒核心物质进入细胞核,启动复制循环。经过隐潜期后病毒复制,在感染24 h后,细胞质中形成病毒发生基质,子代病毒开始形成,逐渐增多。感染48 h后,圆筒形细胞质中的多角体蛋白逐渐沉积并将病毒粒子包埋,最终形成新的多角体。The process of invasion and replication of Bombyx mori cytoplasmic polyhedrosis virus （BmCPV） in the silkworm, Bombyx mor/was studied. After molting, the third instar larvae of B. mori was inoculated with BmCPV then the hind midgut of the infected larvae were dissected at different intervals, prepared as electron microscope specimens and visualized with the transmission electron microscope （TEM）. The results indicated that BmCPV virions were observed associated with the microvilli of the cylindrical cells at 1.5 h post-infection （p. i. ）. The virions entered the microvilli and moved to the cytoplasm 6 h p. i.. The virions were observed in the cylindrical cell at 9 h p. i.. The intact virions probably penetrated through the peritrophic membrane, then absorbed and invaded the microvilli for infecting the cylindrical cells. With the virus degradation, the viral cores went into the nucleus and got under way of multiplication. After the eclipse phase, the new formed virogenic stroma （VS） was found in the cytoplasm of cylindrical cells at 24 h p. i.. Furthermore, the progeny virions appeared and their numbers increased gradually. The polyhedrin deposited to embed the virions to form the polyhedron at 48 h p. i..

关 键 词：家蚕 质型多角体病毒 入侵 复制 

分 类 号：S884.5[农业科学—特种经济动物饲养] Q786[农业科学—畜牧兽医]