香烟及其亚硝胺NNK诱发大鼠气管上皮细胞转化及异硫氰酸衍生物抑制作用的研究  

CIGARETTE SMOKING AND TOEACCO SPECIFIC NI-TROSAMINE NNK INDUCING NEOPLASTIC TRANSFORMA-TION OF RAT TRACHEAL EPITHELIAL CELL AND CHEMO-PREVENTION WITH 6-PHENYLHEXYL ISOTHIOCYANATE

作　　者：王虹[1] 陈雷[1] 项芒 蒋原宙 韩乃君[1] 郭素萍[1] 程书钧[1] 

机构地区：[1]中国医学科学院肿瘤研究所病因室

出　　处：《癌变．畸变．突变》1996年第1期5-9,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：教委博士点基金

摘　　要：利用本室建立的大鼠气管上皮（ＲＴＥ）细胞转化体系，研究了吸烟和肺癌发生的关系以及异硫氰酸衍生物ＰＨＩＴＣ对吸烟诱发肺癌的防护作用。结果表明，香烟烟雾凝集物及其中的亚硝胺ＮＮＫ可以引起ＲＴＥ细胞的转化。采用改进的气管滴注致癌物的方式，较以往降低了ＮＮＫ引起转化的最低济量，有利于正确评估香烟诱发肺癌的危险性。异硫氰醉衍生物ＰＨＩＴＣ可以抑制ＮＮＫ诱导的ＤＮＡ加成物的形成及ＮＮＫ对ＲＴＥ细胞的转化作用。Using rat tracheal epithelial(RTE)cell transformation system, we investigatedthe potential of cigarette smoking to induce neoplastic transformation and the chemopreven-tive effect of 6-phenylhexyl isothiocyanate(PHITC).We found that RTE cells were trans-formed by cigarette smoking condensate(CSC)and tobacco specific notrosamine NNK(4-methylnitrosamino-1-(3-pyridyl)-1-butanone),and PHITC was able to inhibit theNNK-induced DNA alkylation and RTE cell transformation,By directly dripping carcinogeninto rat tracheal epitheliurm,we decreased the minimum dose of NNK inducing transforma-tion.

关 键 词：RTE 肺癌 NNK CSC 异硫氰酸衍生物 

分 类 号：R734.202[医药卫生—肿瘤]