结实期水分胁迫对水稻反义Wx基因转化系主要米质性状及米饭质地的影响  被引量:5

Effects of Water Stress on the Texture of Cooked Rice and the Grain Quality of Transgenic Rice Plants Carrying Antisense Wx Gene during Grain Filling

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作  者:蔡一霞[1] 王维[1] 朱智伟[2] 朱庆森[3] 张祖建[3] 杨建昌[3] 

机构地区:[1]华南农业大学农学院,广东广州510642 [2]中国水稻研究所,浙江杭州310006 [3]扬州大学/江苏省作物遗传生理重点实验室,江苏扬州225009

出  处:《作物学报》2006年第4期475-478,共4页Acta Agronomica Sinica

基  金:财政部农业部跨越计划(1999-3);江苏省作物栽培生理重点实验室开放课题(K04010);华南农业大学博士启动基金(2005K123)

摘  要:以反义Wx基因的转化系及其受体亲本武运粳7号(9522)为材料,研究了结实期水分胁迫对主要品质性状及米饭质地的影响。结果表明,与水层灌溉处理相比,结实期水分胁迫使整精米率和垩白粒率提高;崩解值明显降低,而最终黏度和消减值却明显提高;米饭的硬性增加,黏附性减小,稻米的蒸煮食味品质有着变劣的趋势。It is well known that environments have significant effects on the yield and rice quality. In previous study, the indices of quality, such as chalkiness, gel consistency and RVA profile were ahered by soil water moisture. Amylose content is always used to evaluate the rice eating and cooking quality. And now the characteristics of RVA profile are preferred for the prediction of the texture of cooked rice. Our earlier work showed that RVA profile of rice flour was affected by water stress, but there was no obvious difference in amylose content between well water and water stress. The objective of the study was to research the effects of water stress on the texture of cooked rice and grain quality for rice with the same genetic background. Three transgenic rice lines CA1, CA2, CA3 carrying antisense Wx gene and wild type (cultivar Wuyunjing 7) were grown in pots and were subjected to water stress from flowering stage to filling stage. The results showed that the grain qualities of transgenic rice plants and Wuyunjing 7 were affected by water stress at the grain-filling stage, head milled rice and the percentage of chalky grains increased obviously (Table 3). Changes of RVA profile of rice flour were noticed, breakdown was lower under water stress than under well water, and final viscosity and setback under water stress were higher than those under well water (Table 4). Effect of water stress on the texture of cooked rice presented on the hardness and adhesiveness, the former was increased and the latter was reduced (Table 5), and there was a tendency of low eating and cooking quality.

关 键 词:水分胁迫 稻米品质 米饭质地 反义WX基因 

分 类 号:S511[农业科学—作物学]

 

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