TSHR大分子及其活性多肽在BALB/c小鼠体内的免疫原特性研究  

Characterization of thyrotropin receptor molecule and its active peptide as immunogens in BALB/c mice

作　　者：朱云娟[1] 陈宁[1] 黄丽娟[1] 姚小梅[1] 沈炳玲[1] 叶静[1] 戴玉杰[1] 刘欣[1] 张丽莙[1] 李兰英[1] 陆凤先[1] 

机构地区：[1]天津医科大学病理生理教研室,天津300070

出　　处：《中国病理生理杂志》2006年第4期634-637,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370682);天津市科技发展计划项目(No.05YFGDSF02700)

摘　　要：目的:观察促甲状腺激素受体(TSHR)及其活性片段aa352-366在BALB/c小鼠体内的免疫原特性研究,探讨TSHR分子结构与功能的关系。方法:将血蓝蛋白(KLH)偶联的多肽TSHRaa352-366-KLH和多肽加受体混合组-TSHRaa352-366-KLH加豚鼠TSHR(gTSHR),间隔15d分两组分别注入BALB/c小鼠腹腔内,对照组注射KLH,测定各时相血清中甲状腺激素及促甲状腺激素受体抗体水平;90d后处死动物,检测小鼠甲状腺组织TSHRmRNA水平及其病理变化。结果:与各组自身0d的数据比较,多肽组小鼠血清TT3、TT4水平降低(P<0·01),TRAb升高(P<0·01);多肽加受体混合组TT3、TT4水平降低(P<0·01),TRAb(P<0·01)、TBAb(P<0·05)和TSAb(P<0·05)均升高。此外,混合组甲状腺组织TSHR mRNA水平明显高于正常组(P<0·05);多肽组小鼠甲状腺组织显示滤泡上皮细胞扁平、核缺失、皱缩及胶质浓缩等甲减的病理改变,而混合组则呈现不均一的病理变化。结论:TSHR以及活性片段hTSHRaa352-366都具有免疫原性,刺激小鼠血清TRAb、TSAb和TBAb的产生,引起甲状腺组织的病理改变。AIM： To study the characterization of thyrotropin receptor （TSHR） and its active fragment TSHR aa352- 366 as immunogens in BALB/c mice. METHODS： BALB/c mice were injected peritoneaUy with TSHR aa352 - 366 - KLH （hemocyanin from keyhole limpets） and the mixture of TSHR aa352 - 366 - KLH and guinea pig TSHR every 15 days, respectively. The levels of thyroid hormones and TSHR antibodies and TSHR mRNA were measured, and the pathological changes of thyroid tissue were observed. RESULTS： In the group injected with TSHR aa352 - 366 - KLH, the serum levels of TT3 and TT4 decreased （ P 〈 0.01 ） and levels of TRAb increased （ P 〈 0.01 ）, compared with the data in same group on day 0. The thyroid follicles showed some pathological changes like that of hypothyroidism. In the mixture group, the serum levels of TT3 and TT4 decreased （ P 〈0.01） and levels of TRAb （P〈0.01）, TBAb （P〈0.05） and TSAb （P〈0.05） increased. The levels of TSHR mRNA went up and herterogeneous pathological changes were observed. CONCLUSION： Both gTSHR and hTSHR aa352 - 366 have an immunogenic activity because they induce the production of TRAb, TBAb and TSAb in mice.

关 键 词：受体 促甲状腺素 肽碎片 免疫系统 

分 类 号：R363[医药卫生—病理学]