IL-6基因单核苷酸多态性与腰椎间盘疾病的关联研究  

Association of single nucleotide polymorphism in interleukin 6 gene with lumbar intervebral disc disease

作　　者：叶伟[1] 黄东生[1] 刘尚礼[1] 丁悦[1] 彭焰[1] 胡宝山[1] 吕浩然[1] 

机构地区：[1]中山大学附属第二医院骨科,广东广州510120

出　　处：《中国病理生理杂志》2006年第4期647-651,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助(No.30471746);广东省社会发展计划基金资助(No.2005B34001006);广东省自然科学基金资助(No.5001754)

摘　　要：目的:研究汉族人白细胞介素6(IL-6)基因启动子G-597-A、G-572-C和G-174-C、5号外显子T15-A和C132-T单核苷酸多态性与腰椎间盘疾病关系。方法:采用聚合酶链反应技术,扩增81例腰椎间盘疾病患者和101例正常对照者中分别包含启动子G-597-A、G-572-C、G-174-C和5号外显子T15-A、C132-T位点的片段,直接测序法检测其中的单核苷酸多态性情况,并比较两组中单核苷酸多态性与腰椎间盘疾病的关系。同时,利用MRI检测两组的椎间盘退变情况,并分析其中小于45岁者单核苷酸多态性与椎间盘退变的关系。结果:腰椎间盘疾病病例组及对照组中均未检测到启动子G-597-A和G-174-C、5号外显子T15-A和C132-T单核苷酸多态性情况,仅启动子G-572-C位点存在GG、CG和CC3种表型。但启动子G-572-C单核苷酸多态性与腰椎间盘疾病及椎间盘退变严重程度无关。结论:在汉族人中,IL-6基因仅存在G-572-C单核苷酸多态性,但G-572-C单核苷酸多态性与椎间盘病变及其发病基础椎间盘退变并无关联。AIM： To explore the association of single nucleotide polymorphisms of promoter G^-597 - A, G^-572 - C, G^-174 - C and T^15 - A, C^132 - T in exon 5 of interleukin 6 and lumbar intervebral disc disease. METHODS： The single nucleotide polymorphisms of interleukin 6 gene, including polymorphisms of G^-597 - A, G^-572 - C in promoter, G^-174-- C and T^15 - A, C＆132 - T in exon 5 were analyzed by the polymerase chain reaction and sequencing methods in 81 cases with lumbar intervebral disc disease and 101 healthy controls. The association of single nucleotide polymorphisms of interleukin 6 gene with lumbar intervebral disc disease in two groups was measured. The association of single nucleotide polymorphisms of intedeukin 6 gene with lumbar intervebral disc degeneration in those younger than 45 - year-old were also measured. RESULTS： The G^-572 - C polymorphism of interleukin 6 gene was observed, but no single nucleotide polymorphism of G^-597 -A, G^-174-C in promoter and T^15 - A, C^132 - T in exon 5 in two groups was detected. There was no difference between the distribution of the G^-572- C polymorphism of interleukin 6 gene in two groups. In those younger than 45 - year - old the association of the single nucleotide polymorphism of interluldn - 6 gene and lumbar intervebral disc degeneration was not significant. CONCLUSION： There is G^-572- C polymorphism in Chinese. No relation between G^--72-C polymorphism of interleukin 6 with lumbar intervebral disc disease and lumbar intervebral disc degeneration was observed.

关 键 词：白细胞介素6 核苷酸多态性 腰椎间盘疾病 腰椎间盘退变 

分 类 号：R363[医药卫生—病理学]