甘氨酸脂质体的制备及其对心肌细胞缺氧/复氧损伤的影响  

Preparation of glycine liposomes and it's effect on cardiomyocyte injury induced by hypoxia/reoxygenation

作　　者：陈梦飞[1] 陆大祥[1] 戚仁斌[1] 王华东[1] 王彦平[1] 赵雪[1] 李楚杰[1] 

机构地区：[1]暨南大学医学院病理生理学教研室,广东广州510632

出　　处：《中国病理生理杂志》2006年第4期670-673,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30470718);广东省自然科学基金重点项目(No.04105844);广州市科技局重点项目(No.2004Z3-E4081)

摘　　要：目的:将甘氨酸(Gly)包封于脂质体内,制备甘氨酸脂质体(glycine liposomes)微粒;观察甘氨酸脂质体对培养心肌细胞缺氧/复氧损伤的拮抗作用。方法:(1)采用逆相蒸发法制备Gly脂质体微粒,观察乙醚、氯仿、乙醚+氯仿3种不同有机溶媒对包封率的影响;透射电镜观察Gly脂质体形态、粒径。(2)建立培养乳鼠心肌细胞缺氧/复氧(H/R)损伤模型,实验结束前测定各组培养液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)含量。结果:(1)用乙醚+氯仿混合溶媒制备的Gly脂质体包封率最高,达到64·8%(P<0·01);(2)Gly、Gly脂质体与空白脂质体均能抑制缺氧/复氧诱导的心肌细胞LDH、CK、CK-MB释放。其中,Gly脂质体组抑制作用最为明显(P<0·05,P<0·01)。结论:用乙醚+氯仿混合溶媒制备Gly脂质体能获得较高的包封率;Gly脂质体能对培养乳鼠缺氧/复氧心肌细胞发挥保护作用,其作用机制可能与脂质体携载Gly进入细胞作用于胞内细胞器有关。AIM： To prepare glycine liposome micropartich and observe the effect of glycine liposomes on cardiomyocyte injury induced by hypoxia/reoxygenation. METHODS： （ 1 ） Reverse - phase evaporation method was used to produce glycine liposomes, the effects of different organic solvents： aether, chlroform and two mixtures of aether/chloroform on entrapment efficiency were evaluated, transmission electron microscope was used to detect the particle diameter of glycine liposomes. （2） A cardiomyocyte injury model was established by using hypoxia/reoxygenation, the activities of lactate dehydrogenase （IDH）, creatine kinase （CK） and creatine kinase isoenzyme （CK - MB） of each group were detected. RESULTS： The entrapment efficiency of glycine liposomes prepared with the mixtures of aether/chloroform is highest compared with other organic solvents （64.8%, P 〈0.01）; all of glycine, glycine liposomes and blank liposomes inhibited the release of LDH, CK and CK- MB induced by hypoxia/reoxygenation in cultured cardiomyocytes, and the inhibition by glycine liposomes was the most obvious （P 〈0.05,P 〈0.01）. CONCLUSIONS： The mixtures of aether/chloroform can be used to prepare glycine fiposomes, which can get higher entrapment efficiency; glycine fiposomes protects cultured cardiomyocytes against hypoxia/reoxygenation- induced injury, and the protective effect of glycine fiposomes is better than that of glycine.

关 键 词：甘氨酸 脂质体 心肌细胞 心肌再灌注损伤 

分 类 号：R363[医药卫生—病理学]