LPS促进HUVECs表达纤溶酶原激活物抑制物1  

LPS increases plasminogen activator inhibitor type-1 expression in human endothelial cells

作　　者：唐小龙[1] 江振友[1] 王华东[2] 蔡淑玉[3] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广东广州510632 [2]暨南大学医学院病理生理学教研室,广东广州510632 [3]安徽理工大学医学院,安徽淮南232001

出　　处：《中国病理生理杂志》2006年第4期687-691,共5页Chinese Journal of Pathophysiology

基　　金：广东省医学科研课题(No.A2005340)

摘　　要：目的:观察LPS对脐静脉血管内皮细胞(HUVECs)表达组织纤溶酶原激活物(tPA)和纤溶酶原激活物抑制物1(PAI-1)的影响。方法:用生长良好的第2、3代HUVECs进行试验。用cell counting kit-8(CCK-8)测定LPS刺激后细胞活性变化;发色底物法测定LPS组和对照组培养液中tPA,PAI-1活性;RT-PCR检测细胞内tPA和PAI-1mRNA水平。结果:与对照组相比,LPS(10mg/L)对细胞活性没有明显差异。LPS诱导PAI-1活性在24-72h显著升高(P<0·05),且显著上调PAI-1mRNA,24h达到峰值,以后渐降,72h达到正常水平。而LPS组与对照组tPA活性与tPA mRNA无明显差异(P>0·05)。结论:LPS(10mg/L)可显著上调PAI-1mRNA转录和分泌而不影响tPAmRNA,结果提示LPS可活化内皮细胞,诱发PAI-1mRNA表达和蛋白分泌而抑制纤溶系统,这有利于微血栓的形成、血栓稳定,血液凝固和DIC发生。AIM： The aim of this study is to elucidate the effects of lipopolysaccharide （IPS） on tissue plasminogen activator（tPA） and plssminogen activator inhibitor type - 1 （PAI - 1 ） expression and secretion in endothelial ceils. METHODS： Cultured human umbilical vein endothelial cells （HUVECs） were induced by LPS for different times. Cell viability was then determined by cell counting kit - 8. tPA and PAI - 1 activities in the media were assayed by fibrin overlay and reverse fibrin autograph, respectively. Cytoplasmic RNA was prepared using the Trizol method and was assayed for PAI- 1 and tPA mRNA levels by reverse transcript- polymerase chain reaction（RT- PCR）. RESULTS： IPS（ 10 mg/L） did not produce cell toxicity according to LDH determination in culture media. PAI- 1 activity in IPS group was high （P〈0.05）. Moreover, IPS induced a marked increase in the level of PAl- 1 mRNA till to24h, then began to descend, and down to the normal level at72 h. On the other hand, LPS did not change the level of tPA mRNA and activity （ P 〉 0.05）. CONCLUSION： IPS （ 10 mg/L） did not show signs of cell toxicity, but promoted the expression of PAI - 1 mRNA and induced an increase activity of PAl - 1. However, IPS （ 10 mg/L） did not change tPA mRNA expression. The time- dependent increase in PAI - 1 mRNA expression and activity shifts the local balance toword inclined anti - flbrinolytic capacity, which can amplify the extent of acute thrombosis after plaque rupture. This is one of the possible reasons that cause thrombus,blood coagulation and disseminated intravascular coagulation （DIC） during septicemia.

关 键 词：脂多糖类 组织型纤溶酶原激活物 脐静脉血管内皮细胞 

分 类 号：R363[医药卫生—病理学]