阿魏酸钠抑制纤维粘连蛋白和纤维蛋白原诱导的大鼠血管平滑肌细胞迁移和粘附  

Inhibitory effect of ferulate on attachment and migration induced by fibronectin and fibrinogen in cultured vascular smooth muscle cells from SHR

作　　者：韩英[1] 谢良地[1] 许昌声[1] 王华军[1] 

机构地区：[1]福建省高血压研究所

出　　处：《中国病理生理杂志》2006年第4期703-706,共4页Chinese Journal of Pathophysiology

基　　金：福建省医学创新课题(No.C2003-CX-12)

摘　　要：目的:探讨阿魏酸钠(SF)对纤维粘连蛋白(FN)和纤维蛋白原(Fg)诱导的血管平滑肌细胞(VSMCs)迁移和粘附的影响。方法:VSMCs源于8周龄自发性高血压大鼠(SHR)的胸主动脉,组织块外生法体外培养VSMCs,在96孔聚苯乙烯平底培养板上进行细胞粘附实验;采用改良的Boyden微孔膜双槽法进行细胞迁移实验,荧光染料Fura-2/AM法测定细胞内游离钙离子([Ca2+]i)浓度。结果:(1)FN和Fg可诱导VSMCs的粘附,SF(10-7-10-3mol/L)呈浓度依赖性抑制上述物质诱导的细胞粘附,最大抑制率分别为67·12%和70·23%(P<0·01)。(2)FN和Fg可诱导VSMCs迁移,作用峰值浓度分别为40-60mg/L和1-5g/L。SF(10-7-10-3mol/L)呈浓度依赖性抑制上述物质诱导的细胞迁移,10-3mol/L SF对FN和Fg诱导的细胞迁移的抑制率达69·79%和87·06%。(3)Fg和FN显著促进[Ca2+]i升高(P<0·05),SF明显抑制Fg和FN诱发的[Ca2+]i升高,峰抑制率分别为74·85%和71·57%。结论:Fg和FN可诱导VSMCs粘附、迁移和[Ca2+]i浓度升高,SF可直接抑制VSMCs的粘附、迁移和[Ca2+]i浓度升高,抑制[Ca2+]i浓度升高可能是它抑制上述物质诱导的VSMCs迁移的机制之一。AIM： To investigate the effect of sodium ferulate （ SF） , one of the principal components of rhizoma ligustici wallichii, on the attachment and migration induced by fibronectin （FN） and fibrinogen （Fg） in vascular smooth muscle cells （VSM-Ca）. METHODS： Cultured VSMCa derived from spontaneously hypertensive rats （SHR） were used. Cell attachment was conducted using a flat - bottomed 96 well polystyrene plate. Cell migration was determined by modified Boyden chamber assays. Intracellular free calcium （[Ca^2＋ ]i） was measured with fluorescent Ca^2＋ indicator Fura- 2/AM. RESULTS： FN and Fg signiticanfly induced the attachment and migration of VSMCs in a dose- and time- dependent manner, which was inhibited by pre-treatment of VSMCa with SF （10^-7- 10^-3mol/L） a dose- dependentl fashion. The peak inhibition rate of attachment induced by FN and Fg was 67.12% and 70.23%, respectively, and the peak inhibition rate of SF on migration induced by FN and Fg was 69.79% and 87.06% （ P 〈 0.01 ）. The rise of [ Ca^2＋]i in VSMCs provoked by FN and Fg was significantly suppressed by 10^-3mol/L SF （ P 〈 0.01）. CONCLUSION： The attachment, migration and increase in [Ca^2＋ ]i induced by FN and Fg in VSMCs from SHR are suppressed by SF.

关 键 词：阿魏属 纤维蛋白原 纤维蛋白类 细胞运动 肌 平滑 血管 

分 类 号：R363[医药卫生—病理学]