mic2/CD99在经典型霍奇金淋巴瘤H/RS细胞中的表达及与Eber-1/LMP-1相关性的研究  

Expression of mic2/CD99 protein and their correlation with Eber-1/LMP-1 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma

作　　者：沈丽佳[1] 何滢[1] 蒋会勇[1] 谢思明[1] 朱梅刚[1] 赵彤[1] 

机构地区：[1]南方医科大学南方医院病理科,广东广州510515

出　　处：《中国病理生理杂志》2006年第4期776-780,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.020024)

摘　　要：目的:检测mic2基因与CD99蛋白和Eber-1基因与潜伏膜蛋白-1(LMP1)在经典型霍奇金淋巴瘤(cHL)H/RS细胞中的表达,探讨mic2/CD99表达与Eber-1/LMP-1的相关性。方法:采用分子原位杂交和免疫组化技术并结合组织芯片技术检测59例石蜡包埋淋巴瘤组织标本[43例cHL,16例非霍奇金淋巴瘤(NHL)]mic2/CD99和Eber-1/LMP-1的表达,比较分析mic2/CD99在两组中的表达及与Eber-1/LMP-1的关系。结果:cHL组CD99蛋白表达阳性率为2·3%,mic2基因表达为55·8%,LMP1表达为58·1%,Eber-1表达为53·5%;NHL组CD99蛋白与mic2基因表达高于cHL组(P<0·05);LMP1和Eber-1的表达低于cHL组(P<0·05);mic2基因的表达高于CD99蛋白的表达(P<0·05);Eber-1与LMP1的表达无统计学意义(P>0·05)。CD99蛋白与LMP1的表达呈负相关(P<0·05),各项指标的表达与性别无关。CD99蛋白与LMP1的表达与年龄呈负相关(P<0·05),mic2与Eber-1的表达与年龄无关(P>0·05)。结论:CD99蛋白在cHL H/RS细胞中低表达,并与LMP1的表达呈负相关。AIM： To study the expression of mic2/CD99 protein and their correlation with Eber- 1/LMP- 1 in Hodgkin and Reed - Sternberg cells of classical Hodgkin＇ s lymphoma. METHODS： Immunohistochemical staining, /n situ hybrization and tissue microarry technique were used to detect the expressions of mic2/CD99 and Eber- 1/LMP- 1 of H/RS cells in 43 eases of cHL and 16 cases of NHL. RESU：TS： The positive rate of CD99 protein expression in 43 eases of cHL was 2.3% （1/43）, mie2 was 55.8 % （24/43）, LMP1 was 58.1% （25/43） and Eber - 1 was 53,5 % （23/43）. The expressions of CD99 and mic2 in the NHL group were higher than those in cHL group （ P 〈 0.05）. The expressions of LMP1 and Eber- 1 in NHL group were lower than those in cHL group （ P 〈 0.05）. The expression of mic2 was higher than CD99 in cHL group （ P 〈 0.05）. There was no difference between the expression of Eber - 1 and LMP1 in cHL group statistically （ P 〉 0.05）. There was a negative correlation between the expression of CD99 protein and LMP1 in H/RS cells （ P 〈0.05）. No correlation was found between the expression of any other markers in H/RS cells and patients＇ sex （ P 〉 0.05）. There was a significant correlation between the high expression of LMP1 and a low expression of CD99 in the young patients （ P 〈 0.05）, but no correlation was found between the expression of mic2 and Eber 1 in young patients （ P 〉 0.05）. CONCLUSION： There is a negative correlation between the expression of KMP1 and CD99 in Hodgkin and Reed - Steinberg cells of classical Hodgkin＇ s lymphoma.

关 键 词：霍奇金淋巴瘤 基因 mic2 基因 Eber-1 蛋白质CD99 潜伏膜蛋白质1 

分 类 号：R363[医药卫生—病理学]