全反视黄酸在豚鼠近视形成中的作用  

The role of all-trans retinoic acid in the development of myopia in the guinea pig

作　　者：李瑾[1] 瞿小妹[1] 褚仁远[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院眼科,上海200031

出　　处：《眼视光学杂志》2006年第2期69-72,共4页Chinese Journal of Optometry & Ophthalmology

基　　金：国家自然基金重点项目(30530770)

摘　　要：目的探讨全反视黄酸在豚鼠近视形成中的作用。方法选取4周龄英国短毛豚鼠48只,用0.25%托吡卡胺每5 min散瞳3次,45 min后以带状光检影法测定其屈光不正状态。用康宁动物A超仪(KM-1800)测定玻璃体腔深度和眼轴长。实验组24只随机选取一眼于暗光下球旁注射0.4 g/L浓度全反视黄酸0.2 ml,4周后以同样方法检测豚鼠屈光不正状态、玻璃体腔深度和眼轴长,并取眼球做病理学检查。结果实验组处理前眼屈光不正值为(3.73±0.75)D,玻璃体腔深度为(3.09±0.67)mm,眼轴长为(6.44±0.27)mm;对照组眼屈光不正值为(3.72±0.83)D,玻璃体腔深度为(3.19±0.74)mm,眼轴长为(6.54±0.38)mm,实验组和对照组三项参数比较差异均无显著性(P分别为0.96,0.68及0.26)。4周后对照组屈光不正值为(3.56±0.80)D,玻璃体腔深度为(3.57±0.54)mm,眼轴长为(7.80±0.26)mm,实验处理眼屈光不正值为(0.90±1.25)D,玻璃体腔深度为(3.98±0.68)mm,眼轴长为(8.50±0.39)mm。实验组和对照组三项参数间比较差异有非常显著性(三者均P<0.001)。病理检查提示,实验眼较对照眼相同部位的脉络膜变薄,脉络膜中的黑素细胞增加,色素上皮层粗糙,外丛状层增厚,巩膜层厚度增加。结论局部的全反视黄酸应用可诱导豚鼠近视漂移,提示它是动物模型中近视的信使。它可能首先通过使脉络膜变薄引起玻璃体腔的深度增加,从而致视网膜成像焦点落后导致近视。但增多的视黄酸是近视的起始信使还是近视过程中的产物尚需进一步的研究。Objective To investigate the role of all-trans retinoic acid （RA） in the development of myopia in the guinea pig. Methods Forty-eight pigmented guinea pigs aged 4 weeks were selected for the experiment. Refraction was measured with streak retinoscopy after cycloplegia, ocular leagth and vitreous depth weze calculated with A-scan ultrasonagraphy befoore the start of the. experiment and 4 weeks after 24 guinea pigs were treated with RA. A solution of 0.2 ml RA （0.4 g/L） was injected periglomerularly into one randomly selected eye, and one randomly selected eye in the control group was injected with the same amount of saline and DMSO periglonmeruarly. The eyes were examined under light microscopy to evaluate the pathological changes in the eyes of the RA-treated group and control group. Data was presented as the. mean± SD, differences between the RA-treated group and control group were analy-zed by t-tests, P 〈 0.05 indicated statistically significant differences. Results Eye elongation and vitreous depth gain could be obeyed after four weeks of RA treatment. There were statistically significant differences in the refractive error, ocular length and vitreous depth between the control group and RA-treated group 4 weeks after RA treatment. Before the start of the experiment, refractive statns was （3.73 ± 0.75）D in the RA-treated group and （3.72± 0.83）D in the control group; vitreous depth was （3.09± 0.67）mm and （3.19 ± 0.74）mm, respectively, and ocular length was （6.44 ± 0.27） mm and （6.54 ± 0.38）mm, respectively. Statistcal analysis of these three parameters showed no statistically significant differences between the two groups （P=0.96;P=0.68 and P= 0.26）. Four weeks latex, the refractive status was （3.56± 0.80）D in the control group and （0.90± 1.25）D in the RA-treated group; vitreous depth was （3.57 ± 0.54）mm and （3.98 ± 0.68）mm, respectively; and ocular length was （7.80 ± 0.26）mm and （8.50 ± 0.39）mm, respectively. An analysis of variance ind

关 键 词：全反视黄酸/化学诱导 近视/病因学 豚鼠 

分 类 号：R778.11[医药卫生—眼科]