豚鼠形觉剥夺性近视眼后极部巩膜蛋白多糖水平的变化  

Changes in decorin levels in the posterior sclera of form-deprived guinea pig eyes

作　　者：谭佳[1] 刘双珍[1] 邓志宏[1] 魏欣[1] 

机构地区：[1]中南大学湘雅医院眼科,湖南长沙410008

出　　处：《眼视光学杂志》2006年第2期73-76,共4页Chinese Journal of Optometry & Ophthalmology

摘　　要：目的观察豚鼠形觉剥夺性近视眼模型后极部巩膜蛋白多糖水平的变化，探讨其在哺乳类动物近视眼发生机制中的作用。方法出生2-3周的断乳花色豚鼠20只，随机均分成2组。遮盖组右眼予不透明眼罩遮盖2周，去遮盖组右眼遮盖2周后去遮盖1周。左眼开放为自身对照眼。于实验开始及结束时检影、测眼轴，达规定时限后处死动物，取后极部巩膜，行免疫组织化学及RT-PCR反应，检测decorin核心蛋白及其mRNA的表达。试验数据采用配对t检验争方差分析进行统计学处理。结果豚鼠遮盖组诱导的相对近视约-8．15D。眼轴相对增长约0．63mm；去遮盖组相对近视约-4．30D，眼轴相对增长约0．57mm。去遮盖组屈光程度下降值明显小于遮盖组（F=5.974，P〈0．015）。免疫组织化学法及RT-PCR法示遮盖组和去遮盖组的实验眼和自身对照眼后极部巩膜均有decorin核心蛋白及其mRNA表达；其mRNA相对含量在组内比较差异均有显著性（P〈0．05）。结论豚鼠形觉剥夺性近视眼后极部巩膜decorin mRNA表达显著降低，去除遮盖后其表达上调，提示decorin可能参与了形觉剥夺性近视眼的发生。Objective To investigate the changes in decorin levels in the. posterior sclera of guinea pig eyes induced by form deprivation and its association with the pathogenesis of mammalian myopia. Methods Two groups of guinea pigs, with 10 animals in each group, were included in the study. Animals in the monocular-deprived （MD） group were subjected to 2 weeks of form deprivation, and those in the. MD/recovery group were treated the same way followed by 1 week of recovery. Form deprivation with a monocular occluder was introduced between 2 and 3 weeks of age in all animals. The contralateral, untreated eyes of the animals served as interocular controls. Refraction and axial eye length of all animals were measured at the beginning and the end of the treatment period. All animals were sacrificed and eyes enucleated at the end of study. The posterior sclera was dissected from the eye and the expression of decorin core protein was assayed by immunohistochemistry and RT-PCR. The data were analyzed by a paired t-test and analysis of variance （ANOVA）. Results In the MD group, 2 weeks of monocular deprivation produced - 8.15 D myopia with an 0.63 mm axial elongation of the eye. In the. MD/recovery group, 2 weeks of monocular deprivation followed by 1 week of recovery produced - 4.30 D myopia with an 0.57 nan axial elongation of the eye. There was a significant difference in the amount of myopia between the two groups （ F = 5. 974, P 〈 0.05）. Immunohistachemistry showed positive stains of deceorin core protein in both the MD and control eyes. There were significant differences in the relative levels of decorin core protein mRNA between the MD eyes and interocular controls （P〈 0.05）. This was also observed between the recovered eyes and interocular controls. Conclusion Decoorin levels in the posterior sclexa of guinea pig eyes decrease significantly under form deprivation, but can be. restored during a recovery period. This suggests that decorin might be involved in the pathogenesis of myopia.

关 键 词：豚鼠 近批分析 蛋白多糖/分析 decorin/分析 形觉剥夺 巩膜 

分 类 号：R778.11[医药卫生—眼科] Q539[医药卫生—临床医学]