急性白血病原代细胞TGF-βⅡ型受体同种型的发现及其临床意义  

Identification of the Isoform in Type Ⅱ Receptor of Transforming Growth Factor-beta in Patients with Acute Leukemia and Its Clinical Significance

作　　者：陈萍[1] 陈元仲[1] 吴勇[1] 黄慧芳[1] 李乃农[1] 

机构地区：[1]福建医科大学附属协和医院血液科,福州350004

出　　处：《中国实验血液学杂志》2006年第2期221-224,共4页Journal of Experimental Hematology

基　　金：国家"百千万人才工程"专项基金资助项目

摘　　要：转化生长因子β(TGF-β)及其受体的基因突变已被证实在肿瘤形成中具有重要的作用,在许多肿瘤中都发现有TGF-βⅡ型受体(TβR-Ⅱ)表达异常或表达缺失。为了了解急性白血病患者TβR-Ⅱ基因是否存在异常,本研究应用大范围RT-PCR的方法分段扩增急性白血病原代细胞TβR-Ⅱ的编码序列,并结合序列测定技术,分别对6例急性白血病患者以及11例正常人TβR-Ⅱ的编码序列进行突变检测。全部患者经骨髓细胞形态检查符合FAB诊断标准且骨髓原始细胞≥90%。肝素抗凝骨髓4-5ml,分离单个核细胞,用TRIZOL试剂提取总RNA,反转录后行PCR扩增,琼脂糖凝胶电泳分析PCR产物。回收目的片段,克隆到PGEM-T载体,进行序列的测定。结果显示:在检测的病人中有2例病人中存在TβR-Ⅱ的同种型,而此2例病人临床预后不良。结论:部分白血病病人存在TβR-Ⅱ的同种型,而且该同种型可能与预后有关。Recent research indicates that TGF-β and type Ⅱ receptor （ TβR- Ⅱ ） play an important role in the pathogenesis of tumor. A high frequency of abnormalities in TβR-Ⅱ has been demonstrated in various cancers. To identify the mutation of TβR-Ⅱ in patients with acute leukemia, the bone marrow samples from 6 patients with acute leukemia and 11 normal individuals as control were detected by long-range RT-PCR. To detect a deletion in sequence of the TβR-Ⅱ gene, the PCR products were cloned to T vector and then sequenced. The results showed that there was existance of the isoform of TβR-Ⅱ in 2 cases out of 6 patients with acute leukemia. These two patients had more poor prognosis than others. In conclusion, there was the isoform of TβR-Ⅱ in partial patients with acute leukemia, and the isoform may be related with prognosis.

关 键 词：急性白血病 TβR-Ⅱ 同种型受体 

分 类 号：R733.71[医药卫生—肿瘤]