应用逆转录多重PCR方法检测正常核型急性白血病患者的易位相关融合基因  

Detection of Fusion Genes Associated with Specific Translocations in Acute Leukemia Patients with Normal Karyotypes by Using Multiplex RT-PCR

作　　者：马力[1] 薛永权[2] 潘金兰[2] 何军[2] 吴亚芳[2] 岑建农[2] 温丙昭[1] 

机构地区：[1]新疆医科大学第一附属医院血液科,乌鲁木齐830054 [2]苏州大学第一附属医院,江苏省血液病研究所,苏州215006

出　　处：《中国实验血液学杂志》2006年第2期228-231,共4页Journal of Experimental Hematology

摘　　要：为了探讨逆转录多重PCR方法在检测具有正常核型的急性白血病(AL)患者的易位相关融合基因中的价值,应用包括19种染色体易位特异性引物对的逆转录多重PCR方法对37例经常规细胞遗传学方法揭示为正常核型的AL患者进行了检测。结果表明:有8例(21.6%)AL患者分别检测出有PML/RARA、AML1/ETO、CBFβ/MYH11和BCR/ABL等4种融合基因的存在。结论:逆转录多重PCR可在核型正常的AL患者中检出隐匿的染色体易位,故凡常规细胞遗传学显示为核型正常的AL患者,均应对其进行逆转录多重PCR检测。This study was aimed to explore the usefulness of multiplex reverse transcription-polymerase chain reaction （multiplex RT-PCR）in detection of fusion genes associated with specific translocations in acute leukemia （AL） patients with normal karyotypes. 37 AL patients with normal karyotypes were analyzed by multiplex RT-PCR. The results showed that 4 types of fusion genes such as PML/RARA, AML1/ETO, CBFβ/MYH11, BCR/ABL were detected in 8 （21.6%） patients by multiplex RT-PCR. In conclusion, multiplex RT-PCR is useful in detection of fusion genes associated with specific translocations in acute leukemia （AL） with normal karyotypes and it would refine the karyotype analysis. When the normal karyotypes were detected in acute leukemia patients by conventional cytogenetic method, the multiplex RT-PCR should be performed for them.

关 键 词：逆转录多重PCR 急性白血痛 正常核型 

分 类 号：R733.71[医药卫生—肿瘤]