骨髓增生异常综合征患者间充质干细胞SDF-1基因的表达  

Expression of SDF-1 Gene in Bone Marrow Mesenchymal Stem Cells of Patients with Myelodysplastic Syndrome

作　　者：张翼鷟[1] 达万明[1] 

机构地区：[1]解放军总医院血液科,北京100853

出　　处：《中国实验血液学杂志》2006年第2期281-284,共4页Journal of Experimental Hematology

摘　　要：本研究探讨骨髓基质细胞衍生因子-1(SDF-1)在骨髓增生异常综合征(MDS)患者骨髓间充质干细胞(MSC)中的表达水平。采集MDS的骨髓标本,分离、培养和扩增MSC,观察细胞形态并进行表型鉴定;以实时定量RT-PCR法检测间充质干细胞SDF-1基因和内参照磷酸甘油醛脱氢酶(GAPDH)基因的表达水平,并与健康供者的表达水平相比较。结果发现,MDS患者骨髓间充质干细胞SDF-1基因的表达水平经GAPDH校正后为1.53±0.92,与对照组(5.51±0.99)相比差异非常显著(P<0.01)。结论:SDF-1基因在MDS患者骨髓间充质干细胞中的表达显著升高,SDF-1基因的异常表达可能影响MDS患者骨髓微环境的造血调控作用,是否可对MDS的发病机制及治疗提供新的思路值得进一步探讨。This study was aimed to investigate the expression level of stromal cell derived factor-1 gene （ SDF-1 ） in bone marrow mesenchymal stem ceils （MSC） of patients with myelodysplastic syndrome （MDS）. The MSC from bone marrow samples of MDS patients were isolated, cultured and expanded, the morphology and immunophenotype of MSC were analyzed. The expression levels of SDF-1 and internal reference GAPDH in MSC of MDS patients were detected by real-time quantitative reverse transcriptase polymerase chain reaction （RQ-RT-PCR） method and were compared with expression levels of healthy donors. The results showed that the expression levels of SDF-1 in MDS patients were significantly different from those in healthy donors （ 1.53 ± 0.92 vs 5.51 ± 0. 99 ） （ P 〈 0.01 ）. SDF-1 gene expression levels in bone marrow MSC of MDS patients were significantly higher than that in MSC derived from healthy donors. It is concluded that the abnormal expression of SDF-1 gene in MSC may influence the regulation of hem_atopoiesis of the bone marrow microenvironment in MDS patients and it is worthy of further investigation for new clue on etiological mechanism and treatment of MDS.

关 键 词：骨髓增生异常综合征 间充质干细胞 SDF-1基因 实时定量RT-PCR 

分 类 号：R733.7[医药卫生—肿瘤]